Recombinant Human Netrin-G2 His-tag Protein, CF

R&D Systems | Catalog # 10675-NG

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Netrin-G2 His-tag Protein (10675-NG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q96CW9.2

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Netrin-G2 protein
Asp18-Pro506, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

56 kDa

SDS-PAGE

70-78 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Netrin‑G2 His-Tag protein is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 of 1.5-9 ng/mL.

Scientific Data Images for Recombinant Human Netrin-G2 His-tag Protein, CF

Recombinant Human Netrin-G2 His-tag Protein Binding Activity.

When Recombinant Human Netrin‑G2 His-Tag (Catalog # 10675-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (9375-LR) binds with an ED50 of 1.5-9 ng/mL.

Recombinant Human Netrin-G2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Netrin-G2 His-tag (Catalog # 10675-NG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa.

Formulation, Preparation, and Storage

10675-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-G2

NTNG-2 (Netrin-G2) is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Mature human Netrin-G2 is approximately 490 amino acid (aa) in length. It contains an N-terminal laminin-related region, several epidermal-growth-factor-like domains and a heparin-binding C-terminus that ends in a GPI-linkage (1). The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Mature human Netrin-G2 shares 96% aa identity with mouse and rat Netrin-G2.  NTNG-2 is predominantly expressed in specific neuronal subsets of the developing and mature central nervous system (2). The protein interacts with the extracellular region of their specific Netrin-G ligand receptors LRRC4 (3). The netrin family proteins provide axonal guidance cues during central nervous system development (4).

References

  1. Seiradake E. et al. (2011) The EMBO J. 30:4479.
  2. Pirone A. et al. (2012) PLOS 7(9):e44745.
  3. Woo, J. et al. Mol. Cell Neurosci. (2009) 42:1.
  4. Zhang, Q. et al. (2005) FEBS Lett. 579:3674.

Long Name

Netrin-G2

Alternate Names

Lmnt2, NTNG2

Entrez Gene IDs

84628 (Human)

Gene Symbol

NTNG2

UniProt

Q96CW9.2

Additional Netrin-G2 Products

Product Documents for Recombinant Human Netrin-G2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Netrin-G2 His-tag Protein, CF

For research use only

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