Recombinant Human Neuroligin 4/NLGN4 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (2)
Supplemental Products

Recombinant Human Neuroligin 4/NLGN4 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
Mouse myeloma cell line, NS0-derived human Neuroligin 4X/NLGN4X protein
Gln42-Ser676, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln42 predicted
Predicted Molecular Mass
72.2 kDa
90-100 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Neuroligin 4X/NLGN4X

Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type-B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1 - 3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X-chromosome. It shares 69% - 73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta -neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium-dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8 - 10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).

  1. Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
  2. Chih, B. et al. (2005) Science 307:1324.
  3. Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
  4. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
  5. Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
  6. Fabrichny, I. P. et al. (2007) Neuron 56:979.
  7. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
  8. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  9. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  10. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
  11. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
Entrez Gene IDs
57502 (Human)
Alternate Names
ASPGX2; AUTSX2; HLNX; HNL4X; HNLX; KIAA1260AUTSX2; MGC22376; neuroligin 4, X-linked; Neuroligin 4X; NL4; NLGN; NLGN4; NLGN4neuroligin 4; NLGN4X; X-linked

Citations for Recombinant Human Neuroligin 4/NLGN4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms
    Authors: T Yumoto, M Kimura, R Nagatomo, T Sato, S Utsunomiya, N Aoki, M Kitaura, K Takahashi, H Takemoto, H Watanabe, H Okano, F Yoshida, Y Nao, T Tomita
    Mol Autism, 2020;11(1):68.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay, In Vivo
  2. Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma
    Authors: HS Venkatesh, LT Tam, PJ Woo, J Lennon, S Nagaraja, SM Gillespie, J Ni, DY Duveau, PJ Morris, JJ Zhao, CJ Thomas, M Monje
    Nature, 2017;549(7673):533-537.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human Neuroligin 4/NLGN4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Neuroligin 4/NLGN4 Protein, CF and earn rewards!

Have you used Recombinant Human Neuroligin 4/NLGN4 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review