hh Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF 9119-NL-050: R&D Systems

Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Gln42-Val677, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln42 inferred from enzymatic pyroglutamate treatment revealing Ala43
  • Predicted Molecular Mass
    72 kDa
  • SDS-PAGE
    90-105 kDa, reducing conditions
9119-NL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Neuroligin 4Y/NLGN4Y (Catalog # 9119-NL) Enhances Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Untreated E13 DRG neurons show minimal neurite outgrowth. B) Recombinant Human Neuroligin 4Y/NLGN4Y, immobilized at 10 µg/mL, enhances DRG neuron neurite outgrowth.
Background: Neuroligin 4Y/NLGN4Y
Neuroligin 4 (NLGN4, NL4 or NL4X) is a type I transmembrane molecule in the type-B carboxyesterase/lipase family of proteins. Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of b-neurexin (1). Human NLGN4 maps to the X-chromosome and shares 98% aa identity with the Y-chromosome-encoded NLGN4Y (2). Mature human NLGN4Y consists of a 633 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. It is expressed in the frontal cortex, temporal cortex, occipital cortex, hippocampus, cerebellum, and spinal cord (3, 4). The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (5). Alternative splicing of NLGN4Y generates additional isoforms with variable N-terminal deletions and/or insertions and truncations within the ECD. NLGNs 3, 4, and 4Y bind to the intracellular adaptor protein Syntrophin-g2 (6). Mutations of NLGN4 but not NLGN4Y are associated with rare cases of autism and Asperger or Tourette syndromes (4, 7-12).
  • References:
    1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
    2. Bolliger, M.F. et al. (2001) Biochem. J. 356:581.
    3. Johansson, M.M. et al. (2016) Biol. Sex Differ. 7:5.
    4. Jamain, S. et al. (2003) Nat. Genet. 34:27.
    5. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
    6. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
    7. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
    8. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
    9. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
    10. Yan, J. et al. (2008) Psychiatr. Genet. 18:204.
    11. Serajee, F.J. and A.H. Mahbubul Huq (2009) J. Child Neurol. 24:1258.
    12. Ylisaukko-oja, T. et al. (2005) Eur. J. Hum. Genetics 13:1285.
  • Long Name:
    Neuroligin 4, Y-Linked
  • Entrez Gene IDs:
    22829 (Human)
  • Alternate Names:
    neuroligin 4, Y-linked; Neuroligin 4Y; Neuroligin Y; neuroligin-4, Y-linked; NLGN4Y; Y linked
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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