>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
Chinese Hamster Ovary cell line, CHO-derived Gln42-Val677, with a C-terminal 6-His tag
Recombinant Human Neuroligin 4Y/NLGN4Y (Catalog # 9119-NL) Enhances Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Untreated E13 DRG neurons show minimal neurite outgrowth. B) Recombinant Human Neuroligin 4Y/NLGN4Y, immobilized at 10 µg/mL, enhances DRG neuron neurite outgrowth.
Background: Neuroligin 4Y/NLGN4Y
Neuroligin 4 (NLGN4, NL4 or NL4X) is a
type I transmembrane molecule in the type-B carboxyesterase/lipase family of
proteins. Neuroligins are postsynaptically expressed on neurons and initiate
excitatory presynapse maturation through binding to select isoforms of b-neurexin
(1). Human NLGN4 maps to the X-chromosome and shares 98% aa identity with the
Y-chromosome-encoded NLGN4Y (2). Mature human NLGN4Y consists of a 633 aa
extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa
cytoplasmic tail. It is expressed in the frontal cortex, temporal cortex,
occipital cortex, hippocampus, cerebellum, and spinal cord (3, 4). The ECD
possesses a nonfunctional esterase homology domain through which neuroligins,
except for NLGN2, interact with neurexins (5). Alternative splicing of NLGN4Y
generates additional isoforms with variable N-terminal deletions and/or
insertions and truncations within the ECD. NLGNs 3, 4, and 4Y bind to the intracellular
adaptor protein Syntrophin-g2 (6). Mutations of NLGN4 but not NLGN4Y are
associated with rare cases of autism and Asperger or Tourette syndromes (4,
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Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
Yan, J. et al. (2008) Psychiatr. Genet. 18:204.
Serajee, F.J. and A.H. Mahbubul Huq (2009) J. Child Neurol. 24:1258.
Ylisaukko-oja, T. et al. (2005) Eur. J. Hum. Genetics 13:1285.
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