Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF

Catalog # Availability Size / Price Qty
9119-NL-050
Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF Bioactivity
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Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Neuroligin 4Y/NLGN4Y protein
Gln42-Val677, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln42 inferred from enzymatic pyroglutamate treatment revealing Ala43
Predicted Molecular Mass
72 kDa
SDS-PAGE
90-105 kDa, reducing conditions

Product Datasheets

9119-NL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF Bioactivity View Larger

Recombinant Human Neuroligin 4Y/NLGN4Y (Catalog # 9119-NL) Enhances Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Untreated E13 DRG neurons show minimal neurite outgrowth. B) Recombinant Human Neuroligin 4Y/NLGN4Y, immobilized at 10 µg/mL, enhances DRG neuron neurite outgrowth.

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Background: Neuroligin 4Y/NLGN4Y

Neuroligin 4 (NLGN4, NL4 or NL4X) is a type I transmembrane molecule in the type-B carboxyesterase/lipase family of proteins. Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of b-neurexin (1). Human NLGN4 maps to the X-chromosome and shares 98% aa identity with the Y-chromosome-encoded NLGN4Y (2). Mature human NLGN4Y consists of a 633 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. It is expressed in the frontal cortex, temporal cortex, occipital cortex, hippocampus, cerebellum, and spinal cord (3, 4). The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (5). Alternative splicing of NLGN4Y generates additional isoforms with variable N-terminal deletions and/or insertions and truncations within the ECD. NLGNs 3, 4, and 4Y bind to the intracellular adaptor protein Syntrophin-g2 (6). Mutations of NLGN4 but not NLGN4Y are associated with rare cases of autism and Asperger or Tourette syndromes (4, 7-12).

References
  1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
  2. Bolliger, M.F. et al. (2001) Biochem. J. 356:581.
  3. Johansson, M.M. et al. (2016) Biol. Sex Differ. 7:5.
  4. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  5. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
  6. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
  7. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  8. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
  9. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
  10. Yan, J. et al. (2008) Psychiatr. Genet. 18:204.
  11. Serajee, F.J. and A.H. Mahbubul Huq (2009) J. Child Neurol. 24:1258.
  12. Ylisaukko-oja, T. et al. (2005) Eur. J. Hum. Genetics 13:1285.
Long Name
Neuroligin 4, Y-Linked
Entrez Gene IDs
22829 (Human)
Alternate Names
neuroligin 4, Y-linked; Neuroligin 4Y; Neuroligin Y; neuroligin-4, Y-linked; NLGN4Y; Y linked

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