Recombinant Human NFATC2 Protein, CF

R&D Systems | Catalog # 9716-NA

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human NFATC2 Protein (9716-NA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q13469-1
View all NFATC2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human NFATC2 protein
His575-Pro679, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

13 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9716-NA
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution

Reconstitute at 500 μg/mL in 4 mM HCl..
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NFATC2

Nuclear factor of activated T cells, cystoplamic 2 (NFATC2), also known as T cell transcription factor NFAT1, is a member of the calcium-regulated family of transcription factors that regulate gene expression in lymphocytes and control cardiac valve formation (1, 2). The NFAT family is comprised of four members, NFATC1-C4. Human NFATC2 is a 925 aa transcription factor containing several key regions including a calcineurin-binding site, a trans-activation domain, and nuclear localization and export signals. The first three members of this family are thought to be immune-specific forms of NFAT, while NFATC4 has functions in the regulation of cardiac hypertrophy and hippocampal neuronal signaling (3, 4). In resting T cells, the NFAT proteins are present in the cytoplasm in their phosphorylated form. Upon elevation of intracellular calcium concentration and activation of the phosphatase calcineurin, the NFATC proteins are dephosphorylated and translocated into the nucleus where they regulate the transcription of genes for a number of cytokines and surface molecules (5, 6).

References

  1. Rengarajan, J. et al. (2002) Nat. Immunol. 3:48.
  2. Baksh, S. et al. (2002) Mol. Cell. 10:1071.
  3. Molkentin, J.D. et al. (1998) Cell. 93:215.
  4. Graef, I.A. et al. (1999) Nature. 401:703.
  5. Bopp, T. et al. (2005) J. Exp. Med. 201:181.
  6. Ranger, A.M. et al. (2000) J. Exp. Med. 191:9.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Entrez Gene IDs

4773 (Human)

Gene Symbol

NFATC2

UniProt

Q13469-1

Additional NFATC2 Products

Product Documents for Recombinant Human NFATC2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human NFATC2 Protein, CF

For research use only

Customer Reviews for Recombinant Human NFATC2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human NFATC2 Protein, CF and earn rewards!

Have you used Recombinant Human NFATC2 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes