Recombinant Human NFATC2 Protein, CF
Recombinant Human NFATC2 Protein, CF Summary
His575-Pro679, with an N-terminal Met and a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 500 μg/mL in 4 mM HCl..|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Nuclear factor of activated T cells, cystoplamic 2 (NFATC2), also known as T cell transcription factor NFAT1, is a member of the calcium-regulated family of transcription factors that regulate gene expression in lymphocytes and control cardiac valve formation (1, 2). The NFAT family is comprised of four members, NFATC1-C4. Human NFATC2 is a 925 aa transcription factor containing several key regions including a calcineurin-binding site, a trans-activation domain, and nuclear localization and export signals. The first three members of this family are thought to be immune-specific forms of NFAT, while NFATC4 has functions in the regulation of cardiac hypertrophy and hippocampal neuronal signaling (3, 4). In resting T cells, the NFAT proteins are present in the cytoplasm in their phosphorylated form. Upon elevation of intracellular calcium concentration and activation of the phosphatase calcineurin, the NFATC proteins are dephosphorylated and translocated into the nucleus where they regulate the transcription of genes for a number of cytokines and surface molecules (5, 6).
- Rengarajan, J. et al. (2002) Nat. Immunol. 3:48.
- Baksh, S. et al. (2002) Mol. Cell. 10:1071.
- Molkentin, J.D. et al. (1998) Cell. 93:215.
- Graef, I.A. et al. (1999) Nature. 401:703.
- Bopp, T. et al. (2005) J. Exp. Med. 201:181.
- Ranger, A.M. et al. (2000) J. Exp. Med. 191:9.
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