Recombinant Human Osteopontin (OPN) Protein

Formulations:
Catalog # Availability Size / Price Qty
1433-OP-050
Recombinant Human Osteopontin (OPN) Protein Data
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Product Details
Citations (26)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human Osteopontin (OPN) Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 105 cells/well are added to a Recombinant Human (rh) Osteopontin/OPN coated plate, cell adhesion is enhanced in a
dose-dependent manner after 1 hour incubation at 37 °C.  The ED50  for this effect is 0.1-0.6 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rhOsteopontin/His can increase HEK293 cell adhesion by about 5-fold.
Source
Mouse myeloma cell line, NS0-derived human Osteopontin/OPN protein
Ile17-Asn300, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ile17
Predicted Molecular Mass
32.9 kDa
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1433-OP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1433-OP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human Osteopontin/OPN (Catalog # 1433‑OP), when immobilized, supports the adhesion of the HEK293 human embryonic kidney cells.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human Osteopontin/OPN was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a bands at 60-65 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Human OPN is synthesized as a 317 amino acid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C-terminal CD44 binding site (1-4). Depending on
tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury (6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SVVYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

References
  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Young, M.F. et al. (1990) Genomics 7:491.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

Citations for Recombinant Human Osteopontin (OPN) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. NK cells induce hepatic ER stress to promote insulin resistance in obesity through osteopontin production
    Authors: J Wu, D Wu, L Zhang, C Lin, J Liao, R Xie, Z Li, S Wu, A Liu, W Hu, Y Xi, S Bu, F Wang
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Development of a novel and rapid measurement system for growth differentiation factor-15, progranulin, and osteopontin in uterine sarcoma
    Authors: M Maeno, T Mizutani, H Tsuyoshi, S Yamada, S Ishikane, S Kawabe, K Nishimura, M Yamada, K Miyamoto, Y Yoshida
    Endocr. J., 2019;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: Standard
  3. A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
    Authors: J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang
    Clin Proteomics, 2019;16(0):10.
    Species: Human
    Sample Types: Serum
    Applications: Multiplex Standard
  4. Osteopontin regulates HCV replication and assembly via interaction with HCV proteins and lipid droplets and by binding to receptors, αVβ3 and CD44
    Authors: J Iqbal, M Sarkar-Dut, S McRae, A Ramachandr, B Kumar, G Waris
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion
    Authors: JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Osteopontin mediates glioblastoma-associated macrophage infiltration and is a therapeutic target
    Authors: J Wei, A Marisetty, B Schrand, K Gabrusiewi, Y Hashimoto, M Ott, Z Grami, LY Kong, X Ling, HG Caruso, S Zhou, YA Wang, GN Fuller, JT Huse, E Gilboa, N Kang, X Huang, R Verhaak, S Li, AB Heimberger
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The osteopontin-CD44 axis in hepatic cancer stem cells regulates IFN signaling and HCV replication
    Authors: T Shirasaki, M Honda, T Yamashita, K Nio, T Shimakami, R Shimizu, S Nakasyo, K Murai, N Shirasaki, H Okada, Y Sakai, T Sato, T Suzuki, K Yoshioka, S Kaneko
    Sci Rep, 2018;8(1):13143.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  8. Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
    Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
    Immunol. Cell Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Psoriatic cutaneous inflammation promotes human monocyte differentiation into active osteoclasts, facilitating bone damage
    Authors: A Raimondo, S Lembo, RD Caprio, G Donnarumma, G Monfrecola, N Balato, F Ayala, A Balato
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes
    Authors: Guang-Hua Lei
    Exp Ther Med, 2016;12(5):3488-3494.
    Applications: Bioassay
  11. Trametinib Drives T-cell-Dependent Control of KRAS-Mutated Tumors by Inhibiting Pathological Myelopoiesis
    Cancer Res., 2016;76(21):6253-6265.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Cleaved Form of Osteopontin in Urine as a Clinical Marker of Lupus Nephritis
    PLoS ONE, 2016;11(12):e0167141.
    Species: Human
    Sample Types: Urine
    Applications: ELISA (Standard)
  13. Alcohol inhibits osteopontin-dependent transforming growth factor-beta1 expression in human mesenchymal stem cells.
    Authors: Driver J, Weber C, Callaci J, Kothari A, Zapf M, Roper P, Borys D, Franzen C, Gupta G, Wai P, Zhang J, Denning M, Kuo P, Mi Z
    J Biol Chem, 2015;290(16):9959-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Osteopontin mediates an MZF1-TGF-beta1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.
    Authors: Weber C, Kothari A, Wai P, Li N, Driver J, Zapf M, Franzen C, Gupta G, Osipo C, Zlobin A, Syn W, Zhang J, Kuo P, Mi Z
    Oncogene, 2015;34(37):4821-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. OPN induces FoxM1 expression and localization through ERK 1/2, AKT, and p38 signaling pathway in HEC-1A cells.
    Authors: Xie, Yunpeng, Li, Yinghua, Kong, Ying
    Int J Mol Sci, 2014;15(12):23345-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Role of hepatitis C virus induced osteopontin in epithelial to mesenchymal transition, migration and invasion of hepatocytes.
    Authors: Iqbal J, McRae S, Mai T, Banaudha K, Sarkar-Dutta M, Waris G
    PLoS ONE, 2014;9(1):e87464.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
    Authors: Kato, Aki, Okura, Takafumi, Hamada, Chizuru, Miyoshi, Seigo, Katayama, Hitoshi, Higaki, Jitsuo, Ito, Ryoji
    PLoS ONE, 2014;9(6):e100106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Osteopontin is induced by TGF-beta2 and regulates metabolic cell activity in cultured human optic nerve head astrocytes.
    Authors: Neumann C, Garreis F, Paulsen F, Hammer C, Birke M, Scholz M
    PLoS ONE, 2014;9(4):e92762.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia.
    Authors: Barros N, Hoac B, Neves R, Addison W, Assis D, Murshed M, Carmona A, McKee M
    J Bone Miner Res, 2013;28(3):688-99.
    Species: N/A
    Sample Types: N/A
    Applications: Bioassay
  20. Dual regulation of osteopontin production by TLR stimulation in dendritic cells.
    Authors: Salvi V, Scutera S, Rossi S, Zucca M, Alessandria M, Greco D, Bosisio D, Sozzani S, Musso T
    J Leukoc Biol, 2013;94(1):147-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012;31(0):26.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance
  22. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
    Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2011;52(10):7084-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.
    Authors: Arjomandi M, Frelinger J, Donde A
    PLoS ONE, 2011;6(10):e25678.
    Species: N/A
    Sample Types: N/A
    Applications: Bioassay
  24. Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
    Authors: Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C
    Am. J. Respir. Crit. Care Med., 2009;179(10):894-902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
    Authors: Shao J, Washington MK, Saxena R, Sheng H
    Carcinogenesis, 2007;28(12):2476-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Osteopontin (OPN) Protein
By Anonymous on 08/21/2019
Application: In vitro bioactivity in cell culture