Recombinant Human Osteopontin (OPN) Protein

(17 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 105 cells/well are added to a Recombinant Human (rh) Osteopontin/OPN coated plate, cell adhesion is enhanced in a
    dose-dependent manner after 1 hour incubation at 37 °C.  The ED50  for this effect is 0.1-0.6 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rhOsteopontin/His can increase HEK293 cell adhesion by about 5-fold.
  • Source
    Mouse myeloma cell line, NS0-derived Ile17-Asn300, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ile17
  • Predicted Molecular Mass
    32.9 kDa
  • SDS-PAGE
    60-65 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1433-OP
 
1433-OP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Osteopontin/OPN (Catalog # 1433‑OP), when immobilized, supports the adhesion of the HEK293 human embryonic kidney cells.
1 μg/lane of Recombinant Human Osteopontin/OPN was resolved with
SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a bands at 60-65 kDa.
Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Human OPN is synthesized as a 317 amino acid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C-terminal CD44 binding site (1-4). Depending on
tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury (6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SVVYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

  • References:
    1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
    2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
    3. Young, M.F. et al. (1990) Genomics 7:491.
    4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
    5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
    6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
    7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
    8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
    9. Takafuji, V. et al. (2007) Oncogene 26:6361.
    10. Kiefer, F.W. et al. (2010) Diabetes 59:935.
  • Long Name:
    Secreted Phosphoprotein 1 [BNSP]
  • Entrez Gene IDs:
    6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
  • Alternate Names:
    BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Psoriatic cutaneous inflammation promotes human monocyte differentiation into active osteoclasts, facilitating bone damage
    Authors: A Raimondo, S Lembo, RD Caprio, G Donnarumma, G Monfrecola, N Balato, F Ayala, A Balato
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  2. Trametinib Drives T-cell-Dependent Control of KRAS-Mutated Tumors by Inhibiting Pathological Myelopoiesis
    Cancer Res., 2016;76(21):6253-6265.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Cleaved Form of Osteopontin in Urine as a Clinical Marker of Lupus Nephritis
    PLoS ONE, 2016;11(12):e0167141.
    Species: Human
    Sample Type: Urine
    Application: ELISA Standard
  4. Alcohol inhibits osteopontin-dependent transforming growth factor-beta1 expression in human mesenchymal stem cells.
    Authors: Driver J, Weber C, Callaci J, Kothari A, Zapf M, Roper P, Borys D, Franzen C, Gupta G, Wai P, Zhang J, Denning M, Kuo P, Mi Z
    J Biol Chem, 2015;290(16):9959-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Osteopontin mediates an MZF1-TGF-beta1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.
    Authors: Weber C, Kothari A, Wai P, Li N, Driver J, Zapf M, Franzen C, Gupta G, Osipo C, Zlobin A, Syn W, Zhang J, Kuo P, Mi Z
    Oncogene, 2015;34(37):4821-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Osteopontin is induced by TGF-beta2 and regulates metabolic cell activity in cultured human optic nerve head astrocytes.
    Authors: Neumann C, Garreis F, Paulsen F, Hammer C, Birke M, Scholz M
    PLoS ONE, 2014;9(4):e92762.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
    Authors: Kato, Aki, Okura, Takafumi, Hamada, Chizuru, Miyoshi, Seigo, Katayama, Hitoshi, Higaki, Jitsuo, Ito, Ryoji
    PLoS ONE, 2014;9(6):e100106.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Role of hepatitis C virus induced osteopontin in epithelial to mesenchymal transition, migration and invasion of hepatocytes.
    Authors: Iqbal J, McRae S, Mai T, Banaudha K, Sarkar-Dutta M, Waris G
    PLoS ONE, 2014;9(1):e87464.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. OPN induces FoxM1 expression and localization through ERK 1/2, AKT, and p38 signaling pathway in HEC-1A cells.
    Authors: Xie, Yunpeng, Li, Yinghua, Kong, Ying
    Int J Mol Sci, 2014;15(12):23345-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Dual regulation of osteopontin production by TLR stimulation in dendritic cells.
    Authors: Salvi V, Scutera S, Rossi S, Zucca M, Alessandria M, Greco D, Bosisio D, Sozzani S, Musso T
    J Leukoc Biol, 2013;94(1):147-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia.
    Authors: Barros N, Hoac B, Neves R, Addison W, Assis D, Murshed M, Carmona A, McKee M
    J Bone Miner Res, 2013;28(3):688-99.
    Species: N/A
    Sample Type: N/A
    Application: Activity Assay
  12. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012;31(0):26.
    Species: Human
    Sample Type: Antibody
    Application: Surface Plasmon Resonance
  13. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
    Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2011;52(10):7084-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.
    Authors: Arjomandi M, Frelinger J, Donde A
    PLoS ONE, 2011;6(10):e25678.
    Species: N/A
    Sample Type: N/A
    Application: In vitro polymerization
  15. Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
    Authors: Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C
    Am. J. Respir. Crit. Care Med., 2009;179(10):894-902.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
    Authors: Shao J, Washington MK, Saxena R, Sheng H
    Carcinogenesis, 2007;28(12):2476-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 17 Citations
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag Antibody

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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Human Coagulation Factor II/Thrombin Protein, CF

EnzAct 1473-SE 2
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