Recombinant Human Otolin-1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10234-OT-050
Recombinant Human Otolin-1 His-tag Protein, CF Binding Activity
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Recombinant Human Otolin-1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PLA2G2A (Catalog # 5374-PL) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Otolin-1 His-tag (Catalog # 10234-OT) that produces 50% of the optimal binding response is 20-100 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Otolin-1 protein
Lys24-Pro477, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Lys24
Predicted Molecular Mass
48 kDa
SDS-PAGE
84-94 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10234-OT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human PLA2G2A (Catalog # 5374-PL) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Otolin-1 (Catalog # 10234-OT) binds with an ED50 of 20‑100 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Otolin-1 His-tag (Catalog 10234-OT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-94 kDa and oligomers, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otolin-1

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear (1). It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function (1). It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including and Otoconin-90 (also known as PLA2G2A, PLA2L, and phospholipase A2 homolog) and Cerebellin-1 (1-3). Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus (4). It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions. Human Otolin shares 72% amino acid sequence identity with mouse and rat Otolin.

References
  1. Moreland, K.T. et al. (2014) PloS one 9:e95333.
  2. Zhao, X. et al. (2017) Dev Biol 304:508.
  3. Yang, H. et al. (2011) PLoS ONE, 6(5):e20498.
  4. Tang, Y. T. et al. (2005) Genomics 86:100.
Entrez Gene IDs
131149 (Human); 229389 (Mouse); 295117 (Rat)
Alternate Names
C1q and tumor necrosis factor related protein 15; C1qTNF15; LOC131149; OTOL1; otolin 1; Otolin-1

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