Recombinant Human PBEF/Visfatin Protein, CF

Catalog # Availability Size / Price Qty
8424-VF-050

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Recombinant Human PBEF/Visfatin Protein Bioactivity
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Recombinant Human PBEF/Visfatin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL
Source
E. coli-derived human PBEF/Visfatin protein
Asn2-His491, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
56 kDa
SDS-PAGE
55 kDa, reducing conditions

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8424-VF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8424-VF

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA, TCEP and Tween.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human PBEF/Visfatin Protein Bioactivity View Larger

Recombinant Human PBEF/Visfatin (Catalog # 8424-VF) induces VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL

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Background: PBEF/Visfatin

PBEF, also called Visfatin and NAMPT, is a dimeric type II phosphoribosyltransferase (1). It was initially identified as a secreted cytokine that synergized with IL-7 and SCF to stimulate early stage B cells (2). Human PBEF is predominantly expressed in bone marrow, liver, and muscle, with lower levels found in placenta, kidney, heart, and lung (2). Human PBEF shares 96% and 95% amino acid sequence identity with mouse and rat PBEF, respectively. PBEF has been identified both as an intracellular and as an extracellular protein. Within the cell, PBEF converts nicotinamide to nicotinamide mononucleotide (NMN), which is the rate limiting step in the production of nicotinamide adenine dinucleotide (NAD+) (3, 4). In the extracellular environment, PBEF has been reported to increase the production of inflammatory cytokines and have an important role in the development of T and B cells (5-7). Additionally, PBEF may promote angiogenesis in vivo in an ERK1/2-dependent manner (8). PBEF has been implicated in several inflammatory diseases, including rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, and sepsis (9). PBEF over‑expression has been observed in many cancer types (10).

References
  1. Wang, T. et al. (2006) Nat. Struct. Mol. Biol. 13:661.
  2. Samal, B. et al. (1994) Mol. Cell. Biol. 14:1431.
  3. Rongvaux, A. et al. (2002) Eur. J. Immunol. 32:3225.
  4. Revollo, J.R. et al. (2007) Cell Metab. 6:363.
  5. Ognjanovic, S. and G.D. Bryant-Greenwood (2002) Am. J. Obstet. Gynecol. 187:1051.
  6. Tanaka, M. et al. (2007) Biochem. Biophys. Res. Commun. 359:194.
  7. Rongvaux, A. et al. (2008) J. Immunol. 181:4685.
  8. Kim, S.R. et al. (2007) Biochem. Biophys. Res. Commun. 357:150.
  9. Sun, Z. et al. (2013) Cytokine Growth Factor Rev. 24:433.
  10. Shackelford, R.E. et al. (2013) Genes Cancer 4:447.
Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Entrez Gene IDs
10135 (Human); 59027 (Mouse); 297508 (Rat)
Alternate Names
EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin

Citation for Recombinant Human PBEF/Visfatin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nicotinamide Phosphoribosyltransferase Inhibitor APO866 Prevents IL-1?-Induced Human Nucleus Pulposus Cell Degeneration via Autophagy
    Authors: C Shi, H Wu, D Du, HJ Im, Y Zhang, B Hu, H Chen, X Wang, Y Liu, P Cao, Y Tian, X Shen, R Gao, AJ van Wijnen, X Ye, W Yuan
    Cell. Physiol. Biochem., 2018;49(6):2463-2482.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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