Recombinant Human PBEF/Visfatin Protein, CF

  
  • Purity
    >90%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL
  • Source
    E. coli-derived Asn2-His491, with an N-terminal Met and 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    56 kDa
  • SDS-PAGE
    55 kDa, reducing conditions
8424-VF
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA, TCEP and Tween.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human PBEF/Visfatin (Catalog # 8424-VF) induces VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL
Background: PBEF/Visfatin

PBEF, also called Visfatin and NAMPT, is a dimeric type II phosphoribosyltransferase (1). It was initially identified as a secreted cytokine that synergized with IL-7 and SCF to stimulate early stage B cells (2). Human PBEF is predominantly expressed in bone marrow, liver, and muscle, with lower levels found in placenta, kidney, heart, and lung (2). Human PBEF shares 96% and 95% amino acid sequence identity with mouse and rat PBEF, respectively. PBEF has been identified both as an intracellular and as an extracellular protein. Within the cell, PBEF converts nicotinamide to nicotinamide mononucleotide (NMN), which is the rate limiting step in the production of nicotinamide adenine dinucleotide (NAD+) (3, 4). In the extracellular environment, PBEF has been reported to increase the production of inflammatory cytokines and have an important role in the development of T and B cells (5-7). Additionally, PBEF may promote angiogenesis in vivo in an ERK1/2-dependent manner (8). PBEF has been implicated in several inflammatory diseases, including rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, and sepsis (9). PBEF over‑expression has been observed in many cancer types (10).

  • References:
    1. Wang, T. et al. (2006) Nat. Struct. Mol. Biol. 13:661.
    2. Samal, B. et al. (1994) Mol. Cell. Biol. 14:1431.
    3. Rongvaux, A. et al. (2002) Eur. J. Immunol. 32:3225.
    4. Revollo, J.R. et al. (2007) Cell Metab. 6:363.
    5. Ognjanovic, S. and G.D. Bryant-Greenwood (2002) Am. J. Obstet. Gynecol. 187:1051.
    6. Tanaka, M. et al. (2007) Biochem. Biophys. Res. Commun. 359:194.
    7. Rongvaux, A. et al. (2008) J. Immunol. 181:4685.
    8. Kim, S.R. et al. (2007) Biochem. Biophys. Res. Commun. 357:150.
    9. Sun, Z. et al. (2013) Cytokine Growth Factor Rev. 24:433.
    10. Shackelford, R.E. et al. (2013) Genes Cancer 4:447.
  • Long Name:
    Pre-B-Cell Colony-Enhancing Factor 1
  • Entrez Gene IDs:
    10135 (Human); 59027 (Mouse); 297508 (Rat)
  • Alternate Names:
    EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB, Flow, AP MAB050R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human PBEF/Visfatin Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information