Recombinant Human Periostin/OSF-2 Protein, CF

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Recombinant Human Periostin/OSF-2 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
Mouse myeloma cell line, NS0-derived human Periostin/OSF-2 protein
Asn22-Gln836, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
91.7 kDa
85-90 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

  1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
  2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  7. Li, G. et al. (2006) Atherosclerosis 188:292.
  8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
  10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
  11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
  12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
  13. Snider, P. et al. (2008) Circ. Res. 102:752.
  14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
  15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
  16. Sehra, S. et al. (2011) J. Immunol. 186:4959.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Citations for Recombinant Human Periostin/OSF-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Periostin Augments Vascular Smooth Muscle Cell Calcification via beta-Catenin Signaling
    Authors: I Alesutan, LA Henze, B Boehme, TTD Luong, D Zickler, B Pieske, KU Eckardt, A Pasch, J Voelkl
    Biomolecules, 2022;12(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis
    Authors: Y Ikeda-Iwab, Y Taniyama, N Katsuragi, F Sanada, N Koibuchi, K Shibata, K Shimazu, H Rakugi, R Morishita
    Cells, 2021;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Surface Plasmon Resonance
  3. Smooth muscle-specific MMP17 (MT4-MMP) regulates the intestinal stem cell niche and regeneration after damage
    Authors: M Martín-Alo, S Iqbal, PM Vornewald, HT Lindholm, MJ Damen, F Martínez, S Hoel, A Díez-Sánch, M Altelaar, P Katajisto, AG Arroyo, MJ Oudhoff
    Nature Communications, 2021;12(1):6741.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer
    Authors: H Yamato, K Kimura, E Fukui, T Kanou, N Ose, S Funaki, M Minami, Y Shintani
    Scientific Reports, 2021;11(1):21114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD
    Authors: K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch
    International Journal of Molecular Sciences, 2021;22(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation
    Authors: H Ma, J Wang, X Zhao, T Wu, Z Huang, D Chen, Y Liu, G Ouyang
    Cell Rep, 2020;30(3):793-806.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Periostin plays a critical role in the cell cycle in lung fibroblasts
    Authors: T Yoshihara, Y Nanri, S Nunomura, Y Yamaguchi, C Feghali-Bo, K Ajito, S Murakami, M Mawatari, K Izuhara
    Respir. Res., 2020;21(1):38.
    Species: Human
    Sample Types: cell culture
    Applications: Bioassay
  8. Periostin forms a functional complex with IgA in human serum
    Authors: J Ono, M Takai, A Kamei, S Nunomura, Y Nanri, T Yoshihara, S Ohta, K Yasuda, SJ Conway, Y Yokosaki, K Izuhara
    Allergol Int, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells
    Authors: Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang
    Cell Rep, 2019;26(6):1533-1543.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Fibronectin synthesis, but not ?-smooth muscle expression, is regulated by periostin in gingival healing through FAK/JNK signaling
    Authors: SS Kim, GE Nikoloudak, S Michelsons, K Creber, DW Hamilton
    Sci Rep, 2019;9(1):2708.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin
    Authors: KT Barretto, CM Swanson, CL Nguyen, DS Annis, SJ Esnault, DF Mosher, MW Johansson
    PLoS ONE, 2018;13(7):e0201320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Periostin inhibits mechanical stretch-induced apoptosis in osteoblast-like MG-63�cells
    Authors: KW Yu, CC Yao, JH Jeng, HY Shieh, YJ Chen
    J. Formos. Med. Assoc., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin.
    Authors: Karina T Barretto, Calvin M Swanson, Christopher L Nguyen, Douglas S Annis, Stephane J Esnault, Deane F Mosher, Mats W Johansson
    PLoS ONE, 2018;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Activated hepatic stellate cells secrete periostin to induce stem cell-like phenotype of residual hepatocellular carcinoma cells after heat treatment
    Authors: R Zhang, RR Yao, JH Li, G Dong, M Ma, QD Zheng, DM Gao, JF Cui, ZG Ren, RX Chen
    Sci Rep, 2017;7(1):2164.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration
    Authors: MW Johansson, M Khanna, V Bortnov, DS Annis, CL Nguyen, DF Mosher
    Clin. Exp. Allergy, 2017;0(0):.
    Species: Human
    Sample Types:
    Applications: Bioassay
  16. Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization
    Authors: T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi
    Mol Ther Nucleic Acids, 2017;6(0):279-289.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Periostin Upregulates Wnt/?-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells
    Authors: F Zhang, K Luo, Z Rong, Z Wang, F Luo, Z Zhang, D Sun, S Dong, J Xu, F Dai
    Sci Rep, 2017;7(0):41634.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A study on periostin involvement in the pathophysiology of canine atopic skin
    Authors: T Mineshige, J Kamiie, G Sugahara, K Shirota
    J. Vet. Med. Sci., 2017;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Identification of a novel angiogenic peptide from periostin
    Authors: BR Kim, YW Kwon, GT Park, EJ Choi, JK Seo, IH Jang, SC Kim, HC Ko, SC Lee, JH Kim
    PLoS ONE, 2017;12(11):e0187464.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Identification of axon growth promoters in the secretome of the deer antler velvet
    Neuroscience, 2016;0(0):.
    Species: Red Deer
    Sample Types: Tissue Culture Supernates
    Applications: Bioassay
  21. Identification of axon growth promoters in the secretome of the deer antler velvet
    Authors: Manuel Nieto-Diaz
    Neuroscience, 2016;0(0):.
    Species: Deer
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  22. Upregulation of Periostin and Reactive Stroma Is Associated with Primary Chemoresistance and Predicts Clinical Outcomes in Epithelial Ovarian Cancer.
    Authors: Ryner L, Guan Y, Firestein R, Xiao Y, Choi Y, Rabe C, Lu S, Fuentes E, Huw L, Lackner M, Fu L, Amler L, Bais C, Wang Y
    Clin Cancer Res, 2015;21(13):2941-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Periostin Is a Key Niche Component for Wound Metastasis of Melanoma.
    Authors: Fukuda K, Sugihara E, Ohta S, Izuhara K, Funakoshi T, Amagai M, Saya H
    PLoS ONE, 2015;10(6):e0129704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Inhibition of periostin expression protects against the development of renal inflammation and fibrosis.
    Authors: Mael-Ainin M, Abed A, Conway S, Dussaule J, Chatziantoniou C
    J Am Soc Nephrol, 2014;25(8):1724-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Periostin-binding DNA aptamer inhibits breast cancer growth and metastasis.
    Authors: Lee Y, Kim I, Park S, Kim Y, Lee J, Noh D, Kim K, Ryu S, Suh P
    Mol Ther, 2013;21(5):1004-13.
    Species: Human
    Sample Types: DNA
    Applications: Bioassay
  26. Circulating levels of periostin may help identify patients with more aggressive colorectal cancer.
    Authors: Ben QW, Zhao Z, Ge SF, Zhou J, Yuan F, Yuan YZ
    Int. J. Oncol., 2009;34(3):821-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)


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