Recombinant Human Semaphorin 4D Fc Chimera Protein, CF

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Recombinant Human Semaphorin 4D Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit survival of SK‑OV‑3 human ovarian carcinoma cells. The ED50 for this effect is typically 0.4-2 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 μg/mL, Recombinant Human Semaphorin 4D/CD100 Fc Chimera binds with an apparent Kd <4 nM.
Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 4D/CD100 protein
Human Semaphorin 4D
Accession # Q92854
N-terminus C-terminus
Accession #
N-terminal Sequence
Met22 & Phe24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
105.8 kDa (monomer)
110-135 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D/CD100, previously Sem J, G or C-like 2) is a 150 kDa type I transmembrane glycoprotein that belongs to the Class 4 family of transmembrane immune and nervous system semaphorins (1‑3). The human Sema4D cDNA encodes 862 amino acids (aa) that include a 21 aa signal sequence, a 713 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 107 aa cytoplasmic tail. The Sema4D ECD contains one sema, PSI and immunoglobulin‑like domain, and over the full ECD (aa 22‑734), human Sema4D shares 85%, 85%, 86% and 80% aa sequence identity with mouse, rat, porcine and canine Sema4D ECDs, respectively. Sema4D is active as a homodimer that utilizes both a membrane‑proximal intermolecular disulfide bond and two hydrophobic interactions involving Phe244 and Phe246 (4, 5). Proteolysis by metalloproteinases such as TACE/ADAM17 and MMP-14 (MT1‑MMP) produce a soluble, active 120 kDa form that may also exist as a dimer (5‑7). Sema4D, produced by T cells, activated B cells and dendritic cells acts through a low affinity receptor termed CD72 in the immune system (1, 2). Unligated CD72 inhibits antigen presenting cells that express it, and this inhibition is relieved by Sema4D binding (8). Sema4D thus causes enhanced B cell survival, differentiation of macrophages and DC, and enhanced T cell antigen priming (2, 8, 9). In the CNS, Sema4D and a high affinity receptor Plexin B1 are expressed in complimentary patterns in several regions of the brain, and their interaction assists in the guidance of developing neurons (10). Intermediate affinity Sema4D:Plexin B2 binding also occurs during development, and enhances both radial and tangential neuronal migration (11). In cancer, Sema4D can also be produced by tumor‑associated macrophages, and bind to endothelial cell Plexin B1, thus promoting tumor angiogenesis (3, 6, 7, 12). While this suggests facilitation of the metastatic process, it is also recognized that semaphorins in general show both tumor promoter and suppressor functions (13, 14). For Sema4D, this is reflected in its ability to inhibit ovarian carcinoma cell survival. Finally, receptors that associate in cis with plexins, such as Met and ErbB2, are known to alter Sema4D‑mediated activities (3).

  1. Furuyama, T. et al. (1996) J. Biol. Chem. 271:33376.
  2. Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292.
  3. Ch’ng, E.S. and A. Kumanogoh (2010) Mol. Cancer 9:251.
  4. Janssen, B.J.C. et al. (2010) Nature 467:1118.
  5. Elhabazi, A. et al. (2001) J. Immunol. 166:4341.
  6. Zhu, L. et al. (2007) Proc. Natl. Acad. Sci. USA 104:1621.
  7. Basile, J.R. et al. (2007) J. Biol. Chem. 282:6899.
  8. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  9. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  10. Worzfield, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  11. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  12. Sierra, J.R. et al. (2008) J. Exp. Med. 205:1673.
  13. Ye, S. et al. (2010) BMC Cancer 10:611.
  14. Lu, T-P. et al. (2012) Transl. Cancer Res. 1:74.
Entrez Gene IDs
10507 (Human); 20354 (Mouse); 306790 (Rat)
Alternate Names
A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D

Citations for Recombinant Human Semaphorin 4D Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. An antagonistic monoclonal anti-Plexin-B1 antibody exerts therapeutic effects in mouse models of postmenopausal osteoporosis and multiple sclerosis
    Authors: M Vogler, A Oleksy, S Schulze, M Fedorova, B Kojonazaro, S Nijjar, S Patel, S Jossi, K Sawmynaden, M Henry, R Brown, D Matthews, S Offermanns, T Worzfeld
    The Journal of Biological Chemistry, 2022;0(0):102265.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Neutrophil-Derived Semaphorin 4D Induces Inflammatory Cytokine Production of Endothelial Cells via Different Plexin Receptors in Kawasaki Disease
    Authors: J Huang, S Wu, S Cao, X Zhu, S Zhang
    BioMed Research International, 2020;2020(0):6663291.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Characterization of a novel OTX2-driven stem cell program in Group 3 and Group 4 medulloblastoma
    Authors: M Stromecki, N Tatari, LC Morrison, R Kaur, J Zagozewski, G Palidwor, V Ramaswamy, P Skowron, M Wölfl, T Milde, MR Del Bigio, MD Taylor, TE Werbowetsk
    Mol Oncol, 2018;12(4):495-513.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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