Recombinant Human Protocadherin-18 Protein, CF

Catalog # Availability Size / Price Qty
8315-CA-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Protocadherin-18 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 0.04-0.2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-18 protein
Lys28-Ser699, with a C-terminal 6-­His tag
Accession #
N-terminal Sequence
Analysis
Lys28
Predicted Molecular Mass
75 kDa
SDS-PAGE
90-105 kDa, reducing conditions

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8315-CA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8315-CA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin-18

Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins (1-3). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8+ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).

References
  1. Wolverton, T. and M. Lalande (2001) Genomics 76:66.
  2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.
  3. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
  4. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.
  5. Homayouni, R. et al. (2001) Biochem. Biophys. Res. Commun. 289:539.
  6. Aamar, E. and I.B. Dawid (2008) Dev. Biol. 318:335.
  7. Howell, B.W. et al. (1999) Genes Dev. 13:643.
  8. Howell, B.W. et al. (2000) Curr. Biol. 10:877.
  9. Vazquez-Cintron, E.J. et al. (2012) PLoS One 7:e36101.
  10. Kasnauskiene, J. et al. (2012) Eur. J. Med. Genet. 55:274.
Entrez Gene IDs
54510 (Human); 73173 (Mouse); 295027 (Rat)
Alternate Names
KIAA1562; PCDH68L1; protocadherin 18; Protocadherin18; Protocadherin-18

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