Recombinant Human Protocadherin-18 Protein, CF

Catalog #: 8315-CA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8315-CA-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Protocadherin-18 Protein, CF Summary

R&D Systems CHO-derived Recombinant Human Protocadherin-18 Protein (8315-CA)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.04-0.2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-18 protein
Lys28-Ser699, with a C-terminal 6-His tag

Accession #

Q9HCL0

N-terminal Sequence
Analysis

Lys28

Predicted Molecular Mass

75 kDa

SDS-PAGE

90-105 kDa, reducing conditions

Product Datasheets

8315-CA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8315-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Protocadherin-18

Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins (1-3). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8⁺ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).

References