Recombinant Human Protocadherin-18 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Lys28-Ser699, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins (1-3). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8+ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).
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- Vazquez-Cintron, E.J. et al. (2012) PLoS One 7:e36101.
- Kasnauskiene, J. et al. (2012) Eur. J. Med. Genet. 55:274.
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