Recombinant Human ROR2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
8609-RO-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human ROR2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Measured by its ability to bind biotinylated Recombinant human/mouse Wnt-5a in a functional ELISA. The ED50 for this effect is 1.5-9 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human ROR2 protein
Human ROR2
(Val28-Gly403)
Accession # AAI30523
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
69 kDa
SDS-PAGE
80-92 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8609-RO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROR2

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system (1-5). Mature human ROR2 contains a 369 amino acid (aa) extracellular domain (ECD) and a 518 aa cytoplasmic tail containing an tyrosine kinase domain. The ROR2 ECD features an immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, and a kringle domain. The ECD of human ROR2 shares 93% and 94% aa sequence identity with the ECD of mouse and rat ROR2, respectively. ROR2 binds the Wnt family ligand, Wnt-5a, to activate non-canonical Wnt signaling pathways (6-8). ROR2 is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues (9, 10). Activation of ROR2 signaling promotes cellular proliferation, differentiation, cell-polarization, and migration. ROR2 is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells (11, 12). Mutations in ROR2 are associated with the skeletal disorders, brachydactyly type B and Robinow syndrome (6). ROR2 is also involved in neurite outgrowth and synapse development in the brain (4). A variety of tumors have been found to over-express ROR2. Depending upon the cellular context, ROR2 can act as a tumor suppressor or promoter (13). Expression of ROR2 within carcinomas is correlated with a poor prognosis of survival and increased rates of recurrence (14-16).

References
  1. DeChiara, T.M. et al. (2000) Nat. Genet. 24:271.
  2. Petrova, I.M. et al. (2014) Mol. Neurobiol. 49:303.
  3. Takeuchi, S. et al. (2000) Genes Cells 5:71.
  4. Paganoni, S. and A. Ferreira (2003) J. Neurosci. Res. 73:429.
  5. Endo, M. et al. (2012) J. Cell. Sci. 125:2017.
  6. Minami, Y. et al. (2010) Dev. Dyn. 239:1.
  7. Oishi, I. et al. (2003) Genes Cells 8:645.
  8. Wallkamm, V. et al. (2014) PLoS ONE 9:e109428.
  9. Al-Shawi, R. et al. (2001) Dev. Genes. Evol. 211:161.
  10. Matsuda, T. et al. (2001) Mech. Dev. 105:153.
  11. Cai, S.X. et al. (2014) J. Cell. Physiol. 229:791.
  12. Xin, H. et al. (2013) Int. J. Mol. Med. 31:583.
  13. Ford, C.E. et al. (2013) Int. J. Cancer 133:779.
  14. Rasmussen, N.R. et al. (2014) PLoS ONE 9:e116101.
  15. Mei, H. et al. (2014) Biochem. Biophys. Res. Commun. 453:703.
  16. Lu, B.J. et al. (2012) Mol. Med. Rep. 5:1033.
Long Name
Receptor Tyrosine Kinase-like Orphan Receptor 2
Entrez Gene IDs
4920 (Human); 26564 (Mouse)
Alternate Names
BDB; BDB1; EC 2.7.10.1; MGC163394; neurotrophic tyrosine kinase receptor-related 2; Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2; NTRKR2tyrosine-protein kinase transmembrane receptor ROR2; receptor tyrosine kinase-like orphan receptor 2; ROR2

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