Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).
Recombinant Human SCF, Animal-Free Protein
R&D Systems | Catalog # AFL255
Bigger Scale, More Savings. Try our NEW version of Animal Free Human Recombinant SCF (BT-SCF-AFL). Combining R&D Systems quality with scalability that allows for lower price points and a solid supply chain.
Key Product Details
Learn more about Animal-Free Recombinant Proteins
Source
Accession Number
Applications
Animal Free Proteins
Animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques.
Product Specifications
Source
Glu26-Ala189, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 1‑5 ng/mL.The specific activity of recombinant human SCF is >1.0 x 106 units/mg, which is calibrated against the human SCF reference standard (NIBSC code: 91/682).
Formulation, Preparation, and Storage
AFL255
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: SCF/c-kit Ligand
References
- Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
- Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.
- Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
- Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
- Kapur, R. et al. (2002) Blood 100:1287.
- Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
- Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
- Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
- Martin, F.H. et al. (1990) Cell 63:203.
- Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
- Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
- Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
- Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
- Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
- Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SCF/c-kit Ligand Products
Product Documents for Recombinant Human SCF, Animal-Free Protein
Manufacturing Specifications
Animal-Free Manufacturing ConditionsOur dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Product Specific Notices for Recombinant Human SCF, Animal-Free Protein
For research use or further manufacturing only
Related Research Areas
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