Recombinant Human Semaphorin 3C Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5570-S3-050
Product Details
Citations (1)
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Recombinant Human Semaphorin 3C Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells to Fibronectin. Banu, N. et al. (2006) FASEB J. 20:2150. Recombinant Human (rh) Semaphorin 3C immobilized at 10 μg/mL, with  2 ng/mL rhFN-1 (Catalog # 4305-FNB), 100 μL/well, will induce approximately 50-75% adhesion.
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 3C protein
Human Semaphorin 3C
(Gly21-Ser738: Arg551Ala, Arg552Ala, Arg611Ala, Arg612Ala)
Accession # Q99985
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107.7 kDa (monomer)
SDS-PAGE
110-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5570-S3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Semaphorin 3C

Semaphorin 3C (Sema3C; previously SemaE) is one of six Class 3 secreted semaphorins which share 40 - 50% amino acid (aa) identity among themselves. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 aa human Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1 - 3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Human Sema3C shares at least 95% aa identity with mouse, rat, bovine and canine Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via Neuropilin 1 or Neuropilin 2 (5). Sema3C is expressed in all somitic motor neurons, in lung buds, and in cardiac neural crest cells during development (1, 5 - 8). Sema3C activates integrins in certain cells, so in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin D1 genes or Sema3C neuropilin interactions are disrupted (5, 11, 12).

References
  1. Hinck, L. (2004) Dev. Cell 7:783.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  4. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  5. Gitler, A. D. et al. (2004) Dev. Cell 7:107.
  6. Bagnard, D. et al. (1998) Development 125:5043.
  7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  8. Puschel, A. W. et al. (1995) Neuron 14:941.
  9. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.
  10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
  11. Feiner, L. et al. (2001) Development 128:3061.
  12. Gu, C. et al. (2003) Dev. Cell 5:45.
Entrez Gene IDs
10512 (Human); 20348 (Mouse)
Alternate Names
(semaphorin) 3C; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema E; SEMA3C; SEMAE; Semaphorin 3C; Semaphorin E; semaphorin-3C; semaphorin-E; SEME

Citation for Recombinant Human Semaphorin 3C Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Sema3C Mutant Resistant to Cleavage by Furin (FR-Sema3C) Inhibits Choroidal Neovascularization
    PLoS ONE, 2016;11(12):e0168122.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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