Recombinant Human Siglec-3/CD33 His-tag Protein, CF

R&D Systems | Catalog # 10375-SL

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Siglec-3/CD33 His-tag Protein (10375-SL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P20138.2

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-3/CD33 protein
Met17-His259, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met17

Predicted Molecular Mass

28 kDa

SDS-PAGE

44-49 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.

The ED50 for this effect is 0.1-0.8 µg/mL.

Scientific Data Images for Recombinant Human Siglec-3/CD33 His-tag Protein, CF

Recombinant Human Siglec-3/CD33 His-tag Protein Bioactivity

Recombinant Human Siglec-3/CD33 His-tag Protein Bioactivity

Recombinant Human Siglec-3/CD33 His-tag (Catalog # 10375-SL) supports the adhesion of human red blood cells. The ED50 for this effect is 0.1-0.8 μg/mL.
Recombinant Human Siglec-3/CD33 His-tag Protein SDS-PAGE

Recombinant Human Siglec-3/CD33 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Siglec-3/CD33 His-tag (Catalog # 10375-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-55 kDa.

Formulation, Preparation, and Storage

10375-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-3/CD33

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1-3). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (1, 2). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-3 is alternatively known as myeloid cell surface antigen CD33 and GP67. Human Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (1, 4). Siglec-3 expression is restricted to cells of myelomonocytic lineage (2). It binds sialic acid preferring alpha 2,3-linkage over alpha 2,6-linkage (5). Studies indicated that Siglec-3 recruits SHP-1 and SHP-2 to its ITIMs (6, 7). When co‑cross‑linking with Fc gamma R1, Siglec3- inhibits tyrosine phosphorylation and calcium mobilization, suggesting Siglec-3 can mediate inhibitory signals (7).

References

  1. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  4. Simmons, D. and B. Seed (1988) J. Immunol. 141:2797.
  5. Freeman, S.D. et al. (1995) Blood 85:2002.
  6. Taylor, V.C. et al. (1999) J. Biol. Chem. 274:11505.
  7. Ulyanova, T. et al. (1999) Eur. J. Immunol. 29:3440.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Gene Symbol

CD33

UniProt

P20138.2

Additional Siglec-3/CD33 Products

Product Documents for Recombinant Human Siglec-3/CD33 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Siglec-3/CD33 His-tag Protein, CF

For research use only

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FAQs for Recombinant Human Siglec-3/CD33 His-tag Protein, CF

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  • Q: Is this protein the wild-type or does it contain a mutation at amino acid 69 (R69G)?

    A: The sequence of this protein is Met17-His259, with a C-terminal 6-His tag, and the full referenced sequence for the expressed protein can be found in the Accession section on the product page. This sequence does not contain the R69G natural variant mutation.

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