Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF Summary
Accession # Q9Y286
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.
Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.
- Crocker, P.R. et al. (1998) Glycobiology 8:v.
- Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
- Crocker, P.R. and A. Varki (2001) Immunology 103:137.
- Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
- Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.
Citations for Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
J. Cell. Sci., 2020;0(0):.
Sample Types: Protein
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Sample Types: Cell Lysates
Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion.
Authors: Hudak, Jason E, Canham, Stephen, Bertozzi, Carolyn
Nat Chem Biol, 2014;10(1):69-75.
Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes.
Authors: Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D
PLoS ONE, 2012;7(9):e45821.
Species: Bacteria - E. Coli
Sample Types: Whole Cells
Applications: Binding Assay
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Authors: Zou Z, Chastain A, Moir S
PLoS ONE, 2011;6(9):e24559.
Sample Types: Virus
Applications: Surface Plasmon Resonance
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