Recombinant Human SLAM/CD150 Protein, CF

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164-SL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SLAM/CD150 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.6-2.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human SLAM/CD150 protein
Ala21-Lys236 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala21 & Thr25
Predicted Molecular Mass
25 kDa
SDS-PAGE
40-65 kDa, reducing conditions

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164-SL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

164-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subfamily of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature human SLAM consists of a 217 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, human SLAM shares 58% and 56% aa sequence identity with mouse and rat SLAM, respectively. Alternative splicing generates two additional isoforms, with one showing a deletion in the cytoplasmic region (aa 299-335), and a second showing a deletion in the transmembrane domain (aa 234-263). It is expressed as a 75 kDa molecule, of which approximately 30 kDa represents N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, and it is up-regulated on activated B cells and CD4+ and CD8+ T cells (2‑8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 phenotype in CD8+ T cells that is characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity (2, 3, 9-11). In addition, this interaction on CD4+ T cells promotes a Th2-type response, likely due to an association with the adaptor molecule SAP (4, 12). SLAM ligation also promotes an allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 13-15). In humans, SLAM functions as a cellular entry receptor for measles virus (16, 17).

References
  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
  2. Cocks, B.G. et al. (1995) Nature 376:260.
  3. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  5. Hahm, B. et al. (2004) Virology 323:292.
  6. Nanda, N. et al. (2005) Blood 106:3028.
  7. Kiel, M.J. et al. (2005) Cell 121:1109.
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Davidson, D. et al. (2004) Immunity 21:707.
  13. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  14. Jordan, M.A. et al. (2011) J. Immunol. 186:3953.
  15. Berger, S.B. et al. (2010) Nat. Immunol. 11:920.
  16. Tatsuo, H. et al. (2000) Nature 406:893.
  17. Hsu, E.C. et al. (2001) Virology 279:9.
Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

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