Recombinant Human SLAM/CD150 Protein, CF

R&D Systems | Catalog # 164-SL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human SLAM/CD150 Protein (164-SL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q13291

Applications

Bioactivity
View all SLAM/CD150 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLAM/CD150 protein
Ala21-Lys236 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21 & Thr25

Predicted Molecular Mass

25 kDa

SDS-PAGE

40-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human SLAM/CD150 binds to Biotinylated Recombinant Human SLAM/CD150 with an ED50 of 0.0400-0.400 μg/mL.

Formulation, Preparation, and Storage

164-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subfamily of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature human SLAM consists of a 217 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, human SLAM shares 58% and 56% aa sequence identity with mouse and rat SLAM, respectively. Alternative splicing generates two additional isoforms, with one showing a deletion in the cytoplasmic region (aa 299-335), and a second showing a deletion in the transmembrane domain (aa 234-263). It is expressed as a 75 kDa molecule, of which approximately 30 kDa represents N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, and it is up-regulated on activated B cells and CD4+ and CD8+ T cells (2‑8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 phenotype in CD8+ T cells that is characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity (2, 3, 9-11). In addition, this interaction on CD4+ T cells promotes a Th2-type response, likely due to an association with the adaptor molecule SAP (4, 12). SLAM ligation also promotes an allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 13-15). In humans, SLAM functions as a cellular entry receptor for measles virus (16, 17).

References

  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
  2. Cocks, B.G. et al. (1995) Nature 376:260.
  3. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  5. Hahm, B. et al. (2004) Virology 323:292.
  6. Nanda, N. et al. (2005) Blood 106:3028.
  7. Kiel, M.J. et al. (2005) Cell 121:1109.
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Davidson, D. et al. (2004) Immunity 21:707.
  13. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  14. Jordan, M.A. et al. (2011) J. Immunol. 186:3953.
  15. Berger, S.B. et al. (2010) Nat. Immunol. 11:920.
  16. Tatsuo, H. et al. (2000) Nature 406:893.
  17. Hsu, E.C. et al. (2001) Virology 279:9.

Long Name

Signaling Lymphocytic Activation Molecule

Alternate Names

CD150, IPO-3, SLAMF1

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Gene Symbol

SLAMF1

UniProt

Q13291

Additional SLAM/CD150 Products

Product Documents for Recombinant Human SLAM/CD150 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SLAM/CD150 Protein, CF

For research use only

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