Recombinant Human Slit2 (aa 26-1118) Protein, CF Summary
Gln26-Val1118, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Ethylene Glycol.
|Reconstitution||Reconstitute at 250 μg/mL in water.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is widely expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including seven EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature human Slit2 shares 96% amino acid sequence identity with the mouse and rat orthologs.
Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican-1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to Glypican-1 and promotes motor axon migration (5). Outside the nervous system, Slit2 plays a role in a wide range of biological processes including cell adhesion and migration, tumor progression and metastasis, angiogenesis, lymphangiogenesis, HIV-1 replication, platelet function and thrombus formation, and stem cell senescence (1, 8, 17-30).
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Citations for Recombinant Human Slit2 (aa 26-1118) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils
Authors: D Pilling, LE Chinea, KM Consalvo, RH Gomer
J. Immunol., 2018;0(0):.
Sample Types: Whole Cells
Anthrax edema toxin disrupts distinct steps in Rab11-dependent junctional transport
Authors: A Guichard, P Jain, M Moayeri, R Schwartz, S Chin, L Zhu, B Cruz-Moren, JZ Liu, B Aguilar, A Hollands, SH Leppla, V Nizet, E Bier
PLoS Pathog., 2017;13(9):e1006603.
Sample Types: Whole Cells
Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
Nat Med, 2015;21(5):483-91.
Sample Types: Whole Cells
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