Recombinant Human SLITRK2 Protein, CF

Catalog # Availability Size / Price Qty
8947-SK-050
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Recombinant Human SLITRK2 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived human SLITRK2 protein
Thr19-Pro617, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr19
Predicted Molecular Mass
68 kDa
SDS-PAGE
80-100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8947-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLITRK2

SLITRK2 (Slit and Trk-like family member 2) is an approximately 110 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK2 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK2 shares 98% amino acid sequence identity with mouse and rat SLITRK2. SLITRK2 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (3-5). The synaptogenic function is dependent on the interaction of SLITRK2 with select isoforms of PTP-sigma (5, 6). In humans, mutations of SLITRK6 are associated with bipolar disorder and schizophrenia (7, 8).

References
  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Yamagata, A. et al. (2015) Sci. Rep. 5:9686.
  7. Smith, E.N. et al. (2009) Mol. Pschiatry 14:755.
  8. Piton, A. et al. (2011) Mol. Pschiatry 16:867.
Long Name
SLIT and NTRK-like Family, Member 2
Entrez Gene IDs
84631 (Human); 245450 (Mouse)
Alternate Names
CXorf2; SLITL1; SLITRK2 SLIT and NTRK-like family, member 2; SLITRK2

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