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Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

R&D Systems | Catalog # 1845-SH

GMP Version Available: 1845-GMP
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Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus (1845-SH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_000184.1

Applications

Bioactivity
View all Sonic Hedgehog/Shh Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

20 kDa

SDS-PAGE

22 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.
The ED50 for this effect is 0.1-0.4 µg/mL.

Reviewed Applications

Read 5 reviews rated 4.8 using 1845-SH in the following applications:

Scientific Data Images for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus Protein SEC-MALS.

Recombinant Human SHH Protein (Catalog # 1845-SH) has a molecular weight (MW) of 21.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Bioactivity

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Bioactivity

Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1-0.4 μg/mL.
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus SDS-PAGE

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus SDS-PAGE

1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Mass Spectrometry

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Mass Spectrometry

MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the MALDI-TOF.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1845-SH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1845-SH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

References

  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Gene Symbol

SHH

UniProt

NP_000184.1

Additional Sonic Hedgehog/Shh Products

Product Documents for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1845-SH (with carrier)
Download SDS 1845-SH/CF (carrier free)

Product Specific Notices for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

For research use only

Citations for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Customer Reviews for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus (5)

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  • Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 04/10/2019
  • Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/02/2017
    Granule cells of cerebellum culture for 24 hs in the presence of 100 ng/ml of Shh.
    Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus 1845-SH
  • Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 05/13/2016
  • Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
    Name: Juan Zanin
    Application: Cell Proliferation
    Verified Customer | Posted 05/12/2016
    Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus 1845-SH
  • Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/02/2016

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FAQs for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

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  • Q: What is the difference between Recombinant Human Sonic Hedgehog Catalog # 1845-SH and Catalog # 1314-SH?

    A: Recombinant Human Sonic Hedgehog, Catalog # 1845-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys24-Gly197 (Cys24Ile-Ile), accession number NP_000184. The Cys24Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 1314-SH does not possess this mutation.

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