Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

R&D Systems | Catalog # 174-TC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human TACI/TNFRSF13B Fc Chimera Protein (174-TC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q53F36

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TACI/TNFRSF13B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TACI/TNFRSF13B protein
Human TACI
(Ser2-Thr166)
Accession # Q53F36		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2, Ser123

Predicted Molecular Mass

45.2 kDa (starts at Ser2, monomer)31.3 kDa (starts at Ser123, monomer)

SDS-PAGE

51 kDa and 40 kDa, reducing conditions

Activity

Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
The ED50 for this effect is 3‑10 ng/mL.

Reviewed Applications

Read 5 reviews rated 5 using 174-TC in the following applications:

Formulation, Preparation, and Storage

174-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

  1. Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
  2. von Bulow, G.U. et al. (1997) Science, 278:138.
  3. Gross, J.A. et al. (2000) Nature 404:995.
  4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  5. Yan, M. et al. (2000) Nature Immunol. 1:37.
  6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Alternate Names

CD267, CVID, TNFRSF13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Gene Symbol

TNFRSF13B

UniProt

Q53F36

Additional TACI/TNFRSF13B Products

Product Documents for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

Customer Reviews for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF (5)

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  • Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
    Name: Gerre Lafond
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/19/2021
  • Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/07/2020
  • Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Immunoassay Standard
    Verified Customer | Posted 10/24/2018
  • Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/12/2018
  • Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Apoptosis assay
    Verified Customer | Posted 07/11/2018

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