Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
174-TC-050
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)

Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED50 for this effect is 3‑10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human TACI/TNFRSF13B protein
Human TACI
(Ser2-Thr166)
Accession # Q53F36
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser2, Ser123
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45.2 kDa (starts at Ser2, monomer)31.3 kDa (starts at Ser123, monomer)
SDS-PAGE
51 kDa and 40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

174-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References
  1. Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
  2. von Bulow, G.U. et al. (1997) Science, 278:138.
  3. Gross, J.A. et al. (2000) Nature 404:995.
  4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  5. Yan, M. et al. (2000) Nature Immunol. 1:37.
  6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name
Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Entrez Gene IDs
23495 (Human); 57916 (Mouse)
Alternate Names
CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Citations for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL.
    Authors: Isik G, Sliepen K, van Montfort T, Sanders R
    PLoS ONE, 2014;9(9):e107683.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.
    Authors: Moreaux J, Legouffe E, Jourdan E, Quittet P, Reme T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
    Blood, 2004;103(8):3148-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF

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Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
By Anonymous on 10/24/2018
Application: Immunoassay Standard

Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
By Anonymous on 10/12/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF
By Anonymous on 07/11/2018
Application: Apoptosis assay