Recombinant Human TGF-alpha Protein, CF

Catalog # Availability Size / Price Qty
239-A-100
239-A-500
Product Details
Citations (40)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human TGF-alpha Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is 0.1-0.4 ng/mL.
Source
E. coli-derived human TGF-alpha protein
Val40-Ala89
Accession #
N-terminal Sequence
Analysis
Val40
Predicted Molecular Mass
6 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

239-A

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TGF-alpha

TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.

Long Name
Transforming Growth Factor alpha
Entrez Gene IDs
7039 (Human)
Alternate Names
protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha

Citations for Recombinant Human TGF-alpha Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking
    Authors: L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini
    Cell Rep, 2019;29(7):2067-2077.e6.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. Production of piglets from in�vitro-produced blastocysts by ultrasound-guided ovum pick-up from live donors
    Authors: K Yoshioka, K Uchikura, T Suda, S Matoba
    Theriogenology, 2019;141(0):113-119.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Activating a Reserve Neural Stem Cell Population In�Vitro Enables Engraftment and Multipotency after Transplantation
    Authors: J Peterson, B Lin, CM Barrios-Ca, DB Herrick, EH Holbrook, W Jang, JH Coleman, JE Schwob
    Stem Cell Reports, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  4. Blockage of TGF-? Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells
    Authors: Y Li, H Li, Y Duan, X Cai, D You, F Zhou, C Yang, X Tuo, Z Liu
    Biomed Res Int, 2019;2019(0):8231267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis
    Authors: N Priego, L Zhu, C Monteiro, M Mulders, D Wasilewski, W Bindeman, L Doglio, L Martínez, E Martínez-S, SRY Cajal, D Megías, E Hernández-, C Blanco-Apa, L Martínez, E Zarzuela, J Muñoz, C Fustero-To, E Pineiro, A Hernández-, L Bertero, V Poli, M Sánchez-Ma, JA Menendez, R Soffietti, J Bosch-Barr, M Valiente
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Epiregulin and EGFR interactions are involved in pain processing
    Authors: LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. A loop of cancer-stroma-cancer interaction promotes peritoneal metastasis of ovarian cancer via TNF?-TGF?-EGFR
    Authors: TS Lau, LK Chan, EC Wong, CW Hui, K Sneddon, TH Cheung, SF Yim, JH Lee, CS Yeung, TK Chung, J Kwong
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Negative regulation of the hepatic fibrogenic response by suppressor of cytokine signaling 1
    Cytokine, 2016;82(0):58-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-?-Induced Migration and Invasion of Hepatocellular Carcinoma Cells
    Authors: R Matsushima, H Toyoda, T Nagasawa, E Yasuda, N Chiba, S Okuda, A Maeda, Y Kaneoka, T Kumada, O Kozawa
    PLoS ONE, 2016;11(4):e0151907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. EGFR signaling is critical for maintaining the superficial layer of articular cartilage and preventing osteoarthritis initiation
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14360-14365.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Authors: Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015;135(7):1771-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. RAF inhibitors that evade paradoxical MAPK pathway activation.
    Authors: Zhang C, Spevak W, Zhang Y, Burton E, Ma Y, Habets G, Zhang J, Lin J, Ewing T, Matusow B, Tsang G, Marimuthu A, Cho H, Wu G, Wang W, Fong D, Nguyen H, Shi S, Womack P, Nespi M, Shellooe R, Carias H, Powell B, Light E, Sanftner L, Walters J, Tsai J, West B, Visor G, Rezaei H, Lin P, Nolop K, Ibrahim P, Hirth P, Bollag G
    Nature, 2015;526(7574):583-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015;194(3):1039-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. A comprehensive transcriptional portrait of human cancer cell lines.
    Authors: Klijn C, Durinck S, Stawiski E, Haverty P, Jiang Z, Liu H, Degenhardt J, Mayba O, Gnad F, Liu J, Pau G, Reeder J, Cao Y, Mukhyala K, Selvaraj S, Yu M, Zynda G, Brauer M, Wu T, Gentleman R, Manning G, Yauch R, Bourgon R, Stokoe D, Modrusan Z, Neve R, de Sauvage F, Settleman J, Seshagiri S, Zhang Z
    Nat Biotechnol, 2015;33(3):306-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Signaling by the extracellular matrix protein Reelin promotes granulosa cell proliferation in the chicken follicle.
    Authors: Eresheim, Christin, Leeb, Christia, Buchegger, Patricia, Nimpf, Johannes
    J Biol Chem, 2014;289(14):10182-91.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Authors: Huang, Sarah X, Islam, Mohammad, O'Neill, John, Hu, Zheng, Yang, Yong-Gua, Chen, Ya-Wen, Mumau, Melanie, Green, Michael, Vunjak-Novakovic, Gordana, Bhattacharya, Jahar, Snoeck, Hans-Wil
    Nat Biotechnol, 2014;32(1):84-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Alveolar progenitor and stem cells in lung development, renewal and cancer.
    Authors: Desai, Tushar J, Brownfield, Douglas, Krasnow, Mark A
    Nature, 2014;507(7491):190-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. The Usp8 deubiquitination enzyme is post-translationally modified by tyrosine and serine phosphorylation.
    Authors: Meijer I, Kerperien J, Sotoca A, van Zoelen E, van Leeuwen J
    Cell Signal, 2013;25(4):919-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
    Authors: Kim S, Lewis C, Nadel JA
    J. Immunol., 2011;186(6):3392-400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells.
    Authors: Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M
    Stem Cells, 2011;29(6):964-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Epidermal growth factor receptor reactivation induced by E-prostanoid-3 receptor- and tumor necrosis factor-alpha-converting enzyme-dependent feedback exaggerates interleukin-8 production in airway cancer (NCI-H292) cells.
    Authors: Kim S, Lewis C, Nadel JA
    Exp. Cell Res., 2011;317(18):2650-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010;130(7):1849-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
    Authors: Park JA, Drazen JM, Tschumperlin DJ
    J. Biol. Chem., 2010;285(39):29817-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Ependymal stem cells divide asymmetrically and transfer progeny into the subventricular zone when activated by injury.
    Authors: Gleason D, Fallon JH, Guerra M, Liu JC, Bryant PJ
    Neuroscience, 2008;156(1):81-8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  26. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
    Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
    Clin. Cancer Res., 2008;14(3):840-6.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Developmet
  27. Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.
    Authors: Sasaki T, Nakamura T, Rebhun RB, Cheng H, Hale KS, Tsan RZ, Fidler IJ, Langley RR
    Am. J. Pathol., 2008;173(1):205-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  29. Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
    Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
    Biol. Reprod., 2007;77(1):53-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
    Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
    J. Biol. Chem., 2007;282(37):26656-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006;10(1):39-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
    Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
    Development, 2006;133(4):751-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Cigarette smoke synergistically enhances respiratory mucin induction by proinflammatory stimuli.
    Authors: Baginski TK, Dabbagh K, Satjawatcharaphong C, Swinney DC
    Am. J. Respir. Cell Mol. Biol., 2006;35(2):165-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  35. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. The fibroblast growth factor binding protein is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: implications for epithelial repair.
    Authors: Beer HD, Bittner M, Niklaus G, Munding C, Max N, Goppelt A, Werner S
    Oncogene, 2005;24(34):5269-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Central administration of transforming growth factor-alpha and neuregulin-1 suppress active behaviors and cause weight loss in hamsters.
    Authors: Snodgrass-Belt P, Gilbert JL, Davis FC
    Brain Res., 2005;1038(2):171-82.
    Species: Hamster
    Sample Types: In Vivo
    Applications: In Vivo
  39. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  40. Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
    Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
    Dev. Biol., 2004;266(2):223-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human TGF-alpha Protein, CF
By Anonymous on 05/18/2016
Application: CellProlif
Reason for Rating: This growth factor has been reliable and helped our neural progenitor cells stay healthy and immature.