Recombinant Human Thrombopoietin Protein

Newer Version Available: 288-TPN
NEW
A new economical version of TPO made in NS0 is now available

Carrier Free

Catalog # Availability Size / Price Qty
288-TP-005/CF
288-TP-025/CF
288-TP-01M/CF
288-TP-200/CF

With Carrier

Catalog # Availability Size / Price Qty
288-TP-005
288-TP-025
288-TP-01M
288-TP-200
Recombinant Human Thrombopoietin Protein Bioactivity
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Product Details
Citations (85)
FAQs
Reviews (1)

Recombinant Human Thrombopoietin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE with silver staining
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.3-3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Thrombopoietin/Tpo protein
Ser22-Gly353
Accession #
N-terminal Sequence
Analysis
Ser22
Predicted Molecular Mass
35 kDa
SDS-PAGE
43-60 kDa, reducing conditions

Product Datasheets

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288-TP (with carrier)

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288-TP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

288-TP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

288-TP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50-200 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Thrombopoietin Protein Bioactivity View Larger

Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-3 ng/mL.

SDS-PAGE Recombinant Human Thrombopoietin Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human Thrombopoietin was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 43-60 kDa. Multiple bands in gel are due to variable glycosylation.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10, 13). It is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

References
  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995), Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Citations for Recombinant Human Thrombopoietin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

85 Citations: Showing 1 - 10
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  1. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
    Science Advances, 2022;8(34):eabn5522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis
    Authors: Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura
    Cell Reports, 2022;39(6):110805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A topological refactoring design strategy yields highly stable granulopoietic proteins
    Authors: J Skokowa, B Hernandez, M Coles, M Ritter, M Nasri, J Haaf, N Aghaallaei, Y Xu, P Mir, AC Krahl, KW Rogers, K Maksymenko, B Bajoghli, K Welte, AN Lupas, P Müller, M ElGamacy
    Nature Communications, 2022;13(1):2948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Sequential CRISPR gene editing in human iPSCs charts the clonal evolution of myeloid leukemia and identifies early disease targets
    Authors: T Wang, AR Pine, AG Kotini, H Yuan, L Zamparo, DT Starczynow, C Leslie, EP Papapetrou
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex
    Authors: J Chagraoui, S Girard, JF Spinella, L Simon, E Bonneil, N Mayotte, T MacRae, J Coulombe-H, T Bertomeu, C Moison, E Tomellini, P Thibault, M Tyers, A Marinier, G Sauvageau
    Cell Stem Cell, 2021;28(1):48-62.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes
    Authors: SS Sahoo, VB Pastor, C Goodings, RK Voss, EJ Kozyra, A Szvetnik, P Noellke, M Dworzak, J Starý, F Locatelli, R Masetti, M Schmugge, B De Moerloo, A Catala, K Kállay, D Turkiewicz, H Hasle, J Buechner, K Jahnukaine, M Ussowicz, S Polychrono, OP Smith, O Fabri, S Barzilai, V de Haas, I Baumann, S Schwarz-Fu, European W, MR Niewisch, MG Sauer, B Burkhardt, P Lang, P Bader, R Beier, I Müller, MH Albert, R Meisel, A Schulz, G Cario, PK Panda, J Wehrle, S Hirabayash, M Derecka, R Durruthy-D, G Göhring, A Yoshimi-No, M Ku, D Lebrecht, M Erlacher, C Flotho, B Strahm, CM Niemeyer, MW Wlodarski
    Nature Medicine, 2021;27(10):1806-1817.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells
    Authors: K Yamamoto, S Goyama, S Asada, T Fujino, T Yonezawa, N Sato, R Takeda, A Tsuchiya, T Fukuyama, Y Tanaka, A Yokoyama, H Toya, A Kon, Y Nannya, R Onoguchi-M, S Nakagawa, T Hirose, S Ogawa, N Akimitsu, T Kitamura
    Cell Reports, 2021;36(8):109576.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Base editing of haematopoietic stem cells rescues sickle cell disease in mice
    Authors: GA Newby, JS Yen, KJ Woodard, T Mayuranath, CR Lazzarotto, Y Li, H Sheppard-T, SN Porter, Y Yao, K Mayberry, KA Everette, Y Jang, CJ Podracky, E Thaman, C Lechauve, A Sharma, JM Henderson, MF Richter, KT Zhao, SM Miller, T Wang, LW Koblan, AP McCaffrey, JF Tisdale, TA Kalfa, SM Pruett-Mil, SQ Tsai, MJ Weiss, DR Liu
    Nature, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Study of inherited thrombocytopenia resulting from mutations in ETV6 or RUNX1 using a human pluripotent stem cell model
    Authors: S Borst, CC Nations, JG Klein, G Pavani, JA Maguire, RM Camire, MW Drazer, LA Godley, DL French, M Poncz, P Gadue
    Stem Cell Reports, 2021;16(6):1458-1467.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Generation of disease-specific and CRISPR/Cas9-mediated gene-corrected iPS cells from a patient with adult progeria Werner syndrome
    Authors: H Kato, Y Maezawa, Y Ouchi, N Takayama, M Sone, K Sone, A Takada-Wat, K Tsujimura, M Koshizaka, S Nagasawa, H Saitoh, M Ohtaka, M Nakanishi, H Tahara, A Shimamoto, A Iwama, K Eto, K Yokote
    Stem Cell Research, 2021;53(0):102360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. Endogenous thrombopoietin promotes non-small-cell lung carcinoma cell proliferation and migration by regulating EGFR signalling
    Authors: Z Zou, X Fan, Y Liu, Y Sun, X Zhang, G Sun, X Li, S Xu
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Generation and characterization of HLA-universal platelets derived from induced pluripotent stem cells
    Authors: P Norbnop, P Ingrungrua, N Israsena, K Suphapeeti, V Shotelersu
    Sci Rep, 2020;10(1):8472.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Acute Myeloid Leukemia iPSCs Reveal a Role for RUNX1 in the Maintenance of Human Leukemia Stem Cells
    Authors: J Wesely, AG Kotini, F Izzo, H Luo, H Yuan, J Sun, M Georgomano, A Zviran, AG Deslaurier, N Dusaj, SD Nimer, C Leslie, DA Landau, MG Kharas, EP Papapetrou
    Cell Rep, 2020;31(9):107688.
    Species: Human
    Sample Types:
    Applications: Cell Culture
  14. Thrombopoietin is required for full phenotype expression in a JAK2V617F transgenic mouse model of polycythemia vera
    Authors: JL Spivak, A Merchant, DM Williams, O Rogers, W Zhao, A Duffield, LS Resar, AR Moliterno, ZJ Zhao
    PLoS ONE, 2020;15(6):e0232801.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Homologous Recombination-Based Genome Editing by Clade F AAVs Is Inefficient in the Absence of a Targeted DNA Break
    Authors: GL Rogers, HY Chen, H Morales, PM Cannon
    Mol. Ther., 2019;27(10):1726-1736.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in Mice
    Authors: M Kaur, AC Drake, G Hu, S Rudnick, Q Chen, R Phennicie, R Attar, J Nemeth, F Gaudet, J Chen
    J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2
    Authors: N Uchida, T Nassehi, CM Drysdale, J Gamer, M Yapundich, S Demirci, JJ Haro-Mora, A Leonard, MM Hsieh, JF Tisdale
    Mol Ther Methods Clin Dev, 2019;13(0):187-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Generation of a human induced pluripotent stem cell line, BRCi001-A, derived from a patient with mucopolysaccharidosis type I
    Authors: M Suga, T Kondo, K Imamura, R Shibukawa, Y Okanishi, Y Sagara, K Tsukita, T Enami, M Furujo, K Saijo, Y Nakamura, M Osawa, MK Saito, S Yamanaka, H Inoue
    Stem Cell Res, 2019;36(0):101406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies
    Authors: B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat
    Cell Rep, 2019;27(2):599-615.e12.
    Species: Human
    Sample Types: cell culture
    Applications: Differentiation
  20. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019;130(0):2555-2570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Integrin-&alpha3 Is a Functional Marker of Ex�Vivo Expanded Human Long-Term Hematopoietic Stem Cells
    Authors: E Tomellini, I Fares, B Lehnertz, J Chagraoui, N Mayotte, T MacRae, MÈ Bordeleau, S Corneau, R Bisaillon, G Sauvageau
    Cell Rep, 2019;28(4):1063-1073.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  22. Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells
    Authors: M Ruiz-Gutie, ÖV Bölükba??, G Alexe, AG Kotini, K Ballotti, CE Joyce, DW Russell, K Stegmaier, K Myers, CD Novina, EP Papapetrou, A Shimamura
    JCI Insight, 2019;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  23. Integrin-α3 Is a Functional Marker of Ex�Vivo Expanded Human Long-Term Hematopoietic Stem Cells
    Authors: E Tomellini, I Fares, B Lehnertz, J Chagraoui, N Mayotte, T MacRae, MÈ Bordeleau, S Corneau, R Bisaillon, G Sauvageau
    Cell Rep, 2019;28(4):1063-1073.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  24. CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients
    Authors: M Nasri, M Ritter, P Mir, B Dannenmann, N Aghaallaei, D Amend, V Makaryan, Y Xu, B Fletcher, R Bernhard, I Steiert, K Hahnel, J Berger, I Koch, B Sailer, K Hipp, C Zeidler, M Klimiankou, B Bajoghli, DC Dale, K Welte, J Skokowa
    Haematologica, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Development of a forward-oriented therapeutic lentiviral vector for hemoglobin disorders
    Authors: N Uchida, MM Hsieh, L Raines, JJ Haro-Mora, S Demirci, AC Bonifacino, AE Krouse, ME Metzger, RE Donahue, JF Tisdale
    Nat Commun, 2019;10(1):4479.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  26. NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia
    Authors: FJ Román-Rodr, L Ugalde, L Álvarez, B Díez, MJ Ramírez, C Risueño, M Cortón, M Bogliolo, S Bernal, F March, C Ayuso, H Hanenberg, J Sevilla, S Rodríguez-, R Torres-Rui, J Surrallés, JA Bueren, P Río
    Cell Stem Cell, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. A systems biology pipeline identifies regulatory networks for stem cell engineering
    Authors: MA Kinney, LT Vo, JM Frame, J Barragan, AJ Conway, S Li, KK Wong, JJ Collins, P Cahan, TE North, DA Lauffenbur, GQ Daley
    Nat. Biotechnol., 2019;37(7):810-818.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  28. Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
    Authors: J Grajcarek, J Monlong, Y Nishinaka-, M Nakamura, M Nagai, S Matsuo, D Lougheed, H Sakurai, MK Saito, G Bourque, K Woltjen
    Nat Commun, 2019;10(1):4856.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Low-Dose Busulfan Reduces Human CD34+ Cell Doses Required for Engraftment in c-kit Mutant Immunodeficient Mice
    Authors: A Leonard, M Yapundich, T Nassehi, J Gamer, CM Drysdale, JJ Haro-Mora, S Demirci, MM Hsieh, N Uchida, JF Tisdale
    Mol Ther Methods Clin Dev, 2019;15(0):430-437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium
    Authors: T Fukushima, Y Tanaka, FK Hamey, CH Chang, T Oki, S Asada, Y Hayashi, T Fujino, T Yonezawa, R Takeda, KC Kawabata, T Fukuyama, T Umemoto, K Takubo, H Takizawa, S Goyama, Y Ishihama, H Honda, B Göttgens, T Kitamura
    Cell Rep, 2019;29(12):4144-4158.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  31. UM171 induces a homeostatic inflammatory-detoxification response supporting human HSC self-renewal
    Authors: J Chagraoui, B Lehnertz, S Girard, JF Spinella, I Fares, E Tomellini, N Mayotte, S Corneau, T MacRae, L Simon, G Sauvageau
    PLoS ONE, 2019;14(11):e0224900.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  32. 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia
    Authors: E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert
    Blood Adv, 2018;2(7):754-761.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  33. A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development
    Authors: M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley
    Cell Stem Cell, 2018;22(4):575-588.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. STK3 is a therapeutic target for a subset of acute myeloid leukemias
    Authors: A Camgoz, M Paszkowski, S Satpathy, M Wermke, MV Hamann, M von Bonin, C Choudhary, S Knapp, F Buchholz
    Oncotarget, 2018;9(39):25458-25473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Molecular transitions in early progenitors during human cord blood hematopoiesis
    Authors: S Zheng, E Papalexi, A Butler, W Stephenson, R Satija
    Mol. Syst. Biol., 2018;14(3):e8041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment
    Authors: X Huang, B Guo, S Liu, J Wan, HE Broxmeyer
    Nat Commun, 2018;9(1):2741.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Brief Report: Human Acute Myeloid Leukemia Reprograming to Pluripotency Is a Rare Event and Selects for Patient Hematopoietic Cells Devoid of Leukemic Mutations
    Authors: JH Lee, KR Salci, JC Reid, L Orlando, B Tanasijevi, Z Shapovalov, M Bhatia
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
    Authors: AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Haematopoietic stem and progenitor cells from human pluripotent stem cells
    Authors: R Sugimura, DK Jha, A Han, C Soria-Vall, EL da Rocha, YF Lu, JA Goettel, E Serrao, RG Rowe, M Malleshaia, I Wong, P Sousa, TN Zhu, A Ditadi, G Keller, AN Engelman, SB Snapper, S Doulatov, GQ Daley
    Nature, 2017;545(7655):432-438.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens
    Authors: I Baccelli, J Krosl, G Boucher, I Boivin, VP Lavallée, J Hébert, S Lemieux, A Marinier, G Sauvageau
    Blood Cancer J, 2017;7(2):e529.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease
    Authors: J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin
    Mol Ther Methods Clin Dev, 2017;5(0):241-258.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Clinical efficacy of gene-modified stem cells in adenosine deaminase-deficient immunodeficiency
    Authors: KL Shaw, E Garabedian, S Mishra, P Barman, A Davila, D Carbonaro, S Shupien, C Silvin, S Geiger, B Nowicki, EM Smogorzews, B Brown, X Wang, S de Oliveir, Y Choi, A Ikeda, D Terrazas, PY Fu, A Yu, BC Fernandez, AR Cooper, B Engel, G Podsakoff, A Balamuruga, S Anderson, L Muul, GJ Jagadeesh, N Kapoor, J Tse, TB Moore, K Purdy, R Rishi, K Mohan, S Skoda-Smit, D Buchbinder, RS Abraham, A Scharenber, OO Yang, K Cornetta, D Gjertson, M Hershfield, R Sokolic, F Candotti, DB Kohn
    J. Clin. Invest, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes
    Authors: A Palau, AK Garz, J Diesch, A Zwick, R Malinverni, V Valero, K Lappin, R Casquero, A Lennartsso, J Zuber, T Navarro, KI Mills, KS Götze, M Buschbeck
    Oncotarget, 2017;8(70):115002-115017.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis
    Authors: NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry
    Cell Rep, 2017;20(7):1597-1608.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment
    Authors: B Guo, X Huang, S Cooper, HE Broxmeyer
    Nat. Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. MicroRNA-486-5p is an erythroid oncomiR of the myeloid leukemias of Down syndrome.
    Authors: Shaham L, Vendramini E, Ge Y, Goren Y, Birger Y, Tijssen M, McNulty M, Geron I, Schwartzman O, Goldberg L, Chou S, Pitman H, Weiss M, Michaeli S, Sredni B, Gottgens B, Crispino J, Taub J, Izraeli S
    Blood, 2015;125(8):1292-301.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Small-molecule inhibitor of glycogen synthase kinase 3beta 6-Bromoindirubin-3-oxime inhibits hematopoietic regeneration in stem cell recipient mice.
    Authors: Shen S, Xu N, Klamer G, Ko K, Khoo M, Ma D, Moore J, O'Brien T, Dolnikov A
    Stem Cells Dev, 2015;24(6):724-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Enrichment of human hematopoietic stem/progenitor cells facilitates transduction for stem cell gene therapy.
    Authors: Baldwin K, Urbinati F, Romero Z, Campo-Fernandez B, Kaufman M, Cooper A, Masiuk K, Hollis R, Kohn D
    Stem Cells, 2015;33(5):1532-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Regulated apoptosis of genetically modified hematopoietic stem and progenitor cells via an inducible caspase-9 suicide gene in rhesus macaques.
    Authors: Barese C, Felizardo T, Sellers S, Keyvanfar K, Di Stasi A, Metzger M, Krouse A, Donahue R, Spencer D, Dunbar C
    Stem Cells, 2015;33(1):91-100.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Prospective isolation of human erythroid lineage-committed progenitors.
    Authors: Mori Y, Chen J, Pluvinage J, Seita J, Weissman I
    Proc Natl Acad Sci U S A, 2015;112(31):9638-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
    Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
    Mol Ther, 2015;23(2):339-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
    Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
    PLoS ONE, 2015;10(4):e0122983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
    Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
    Blood, 2014;123(5):650-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells.
    Authors: Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K
    Cell Stem Cell, 2014;14(4):535-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Epigenetic reprogramming induces the expansion of cord blood stem cells.
    Authors: Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R
    J Clin Invest, 2014;124(6):2378-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
    Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
    Blood, 2014;123(8):1239-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Evaluation of engraftment and immunological tolerance after reduced intensity conditioning in a rhesus hematopoietic stem cell gene therapy model.
    Authors: Uchida N, Weitzel R, Evans M, Green R, Bonifacino A, Krouse A, Metzger M, Hsieh M, Donahue R, Tisdale J
    Gene Ther, 2014;21(2):148-57.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP).
    Authors: Rosen, David B, Leung, Ling Y, Louie, Brent, Cordeiro, James A, Conroy, Andrew, Shapira, Iuliana, Fields, Scott Z, Cesano, Alessand, Hawtin, Rachael
    J Transl Med, 2014;12(0):184.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Clonal tracking of rhesus macaque hematopoiesis highlights a distinct lineage origin for natural killer cells.
    Authors: Wu C, Li B, Lu R, Koelle S, Yang Y, Jares A, Krouse A, Metzger M, Liang F, Lore K, Wu C, Donahue R, Chen I, Weissman I, Dunbar C
    Cell Stem Cell, 2014;14(4):486-99.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies.
    Authors: Kazuki, Yasuhiro, Yakura, Yuwna, Abe, Satoshi, Osaki, Mitsuhik, Kajitani, Naoyo, Kazuki, Kanako, Takehara, Shoko, Honma, Kazuhisa, Suemori, Hirofumi, Yamazaki, Satoshi, Sakuma, Tetsushi, Toki, Tsutomu, Shimizu, Ritsuko, Nakauchi, Hiromits, Yamamoto, Takashi, Oshimura, Mitsuo
    Sci Rep, 2014;4(0):6136.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Intracellular reactive oxygen species mark and influence the megakaryocyte-erythrocyte progenitor fate of common myeloid progenitors.
    Authors: Shinohara A, Imai Y, Nakagawa M, Takahashi T, Ichikawa M, Kurokawa M
    Stem Cells, 2014;32(2):548-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  64. MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells.
    Authors: Kamat V, Paluru P, Myint M, French D, Gadue P, Diamond S
    Stem Cells, 2014;32(5):1337-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Allelic exclusion and peripheral reconstitution by TCR transgenic T cells arising from transduced human hematopoietic stem/progenitor cells.
    Authors: Giannoni F, Hardee C, Wherley J, Gschweng E, Senadheera S, Kaufman M, Chan R, Bahner I, Gersuk V, Wang X, Gjertson D, Baltimore D, Witte O, Economou J, Ribas A, Kohn D
    Mol Ther, 2013;21(5):1044-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. beta-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model.
    Authors: Sjeklocha L, Wong P, Belcher J, Vercellotti G, Steer C
    PLoS ONE, 2013;8(11):e80403.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  67. Exome sequencing reveals a thrombopoietin ligand mutation in a Micronesian family with autosomal recessive aplastic anemia.
    Authors: Dasouki, Majed J, Rafi, Syed K, Olm-Shipman, Adam J, Wilson, Nathan R, Abhyankar, Sunil, Ganter, Brigitte, Furness, L Mike, Fang, Jianwen, Calado, Rodrigo, Saadi, Irfan
    Blood, 2013;122(20):3440-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  68. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors.
    Authors: Doulatov S, Vo L, Chou S, Kim P, Arora N, Li H, Hadland B, Bernstein I, Collins J, Zon L, Daley G
    Cell Stem Cell, 2013;13(4):459-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Epigenetic regulation of NANOG by miR-302 cluster-MBD2 completes induced pluripotent stem cell reprogramming.
    Authors: Lee M, Prasain N, Chae H, Kim Y, Mantel C, Yoder M, Broxmeyer H
    Stem Cells, 2013;31(4):666-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages.
    Cell Death Differ., 2012;19(11):1856-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. Targeting of CD34+CD38- cells using Gemtuzumab ozogamicin (Mylotarg) in combination with tipifarnib (Zarnestra) in Acute Myeloid Leukaemia.
    BMC Cancer, 2012;12(0):431.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Treatment of canine leukocyte adhesion deficiency by foamy virus vectors expressing CD18 from a PGK promoter.
    Authors: Bauer TR, Olson EM, Huo Y
    Gene Ther., 2011;18(6):553-9.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  74. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  75. Refreezing of cord blood hematopoietic stem cells for allogenic transplantation: in vitro and in vivo validation of a clinical phase I/II protocol in European and Italian Good Manufacturing Practice conditions.
    Authors: Gunetti M, Ferrero I, Rustichelli D, Berger M, Gammaitoni L, Timeus F, Piacibello W, Aglietta M, Fagioli F
    Exp. Hematol., 2008;36(2):235-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. High thrombopoietin concentrations in the cerebrospinal fluid of neonates with sepsis and intraventricular hemorrhage may contribute to brain damage.
    Authors: Reinhold A, Zhang J, Gessner R, Felderhoff-Mueser U, Obladen M, Dame C
    J. Interferon Cytokine Res., 2007;27(2):137-45.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  78. Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
    Authors: Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM
    Arthritis Rheum., 2007;56(7):2432-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  79. Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells.
    Authors: Srivastava AS, Nedelcu E, Esmaeli-Azad B, Mishra R, Carrier E
    Stem Cells, 2007;25(6):1456-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  80. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006;13(10):1686-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  81. Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells.
    Authors: Kurita R, Sasaki E, Yokoo T, Hiroyama T, Takasugi K, Imoto H, Izawa K, Dong Y, Hashiguchi T, Soda Y, Maeda T, Suehiro Y, Tanioka Y, Nakazaki Y, Tani K
    Stem Cells, 2006;24(9):2014-22.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Types: Whole Cells
    Applications: Bioassay
  82. Circulating thrombopoietin as an in vivo growth factor for blast cells in acute myeloid leukemia.
    Authors: D&amp;amp;apos;Hondt S
    Blood, 2006;107(6):2525-30.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Control)
  83. Effects of busulfan dose escalation on engraftment of infant rhesus monkey hematopoietic stem cells after gene marking by a lentiviral vector.
    Authors: Kahl CA, Tarantal AF, Lee CI, Jimenez DF, Choi C, Pepper K, Petersen D, Fletcher MD, Leapley AC, Fisher J, Burns TS, Ultsch MN, Dorey FJ, Kohn DB
    Exp. Hematol., 2006;34(3):369-81.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  84. Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
    Authors: Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y
    Exp. Hematol., 2006;34(6):760-9.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  85. The reduction of in vitro radiation-induced Fas-related apoptosis in CD34+ progenitor cells by SCF, FLT-3 ligand, TPO, and IL-3 in combination resulted in CD34+ cell proliferation and differentiation.
    Authors: Drouet M, 49153, Mathieu J, Grenier N, Multon E, Sotto JJ, Herodin F
    Circulating NOD1 Activators and Hematopoietic NOD1 Contribute to Metabolic Inflammation and Insulin Resistance, 1999;17(5):273-85.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Why do you sell Thrombopoietin protein from two different expression systems?  Under what circumstances would it be preferred to use the NS0-expressed protein?

    • The NS0-expressed protein is necessary for the serum-free megakaryocyte colony stimulating assay (Nadine Weich, Genetics Insititute).  In all other assays, it works the same as the Sf-21 derived protein.

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Recombinant Human Thrombopoietin Protein
By Anonymous on 10/16/2020
Application: CellProlif

This product is perfect for culturing megakaryocyte progenitor cells.