Recombinant Human TMIGD2/CD28H His-tag Protein, CF

Catalog # Availability Size / Price Qty
9236-TR-050
Recombinant Human TMIGD2/CD28H His-tag Protein, CF Bioactivity
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Recombinant Human TMIGD2/CD28H His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TMIGD2/CD28H is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human
B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) that produces 50% of the optimal binding response is approximately 0.2-1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TMIGD2/CD28H protein
Leu23-Gly150, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu23
Structure / Form


Predicted Molecular Mass
15 kDa
SDS-PAGE
21-34 kDa, reducing conditions

Product Datasheets

9236-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human TMIGD2/CD28H His-tag Protein, CF Bioactivity View Larger

When Recombinant Human TMIGD2/CD28H (Catalog # 9236-TR) isimmobilized at 0.125 μg/mL, Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) binds with an ED50 of
0.2-1 μg/mL.

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Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References
  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
Long Name
Transmembrane And Immunoglobulin Domain Containing 2
Entrez Gene IDs
126259 (Human)
Alternate Names
CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2

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