Recombinant Human TMIGD2/CD28H His-tag Protein, CF Summary
B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) that produces 50% of the optimal binding response is approximately 0.2-1 μg/mL.
Leu23-Gly150, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human TMIGD2/CD28H (Catalog # 9236-TR) is immobilized at 0.125 µg/mL, Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) binds with an ED50 of 0.2-1 µg/mL.
CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).
- Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
- Parry, R.V. et al. (2003) J.Immunol. 171:166.
- Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
- Huang, C. et al. (2003) Nature 424:219.
- Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
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