Recombinant Human TMIGD2/CD28H His-tag Protein, CF

R&D Systems | Catalog # 9236-TR

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human TMIGD2/CD28H His-tag Protein (9236-TR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q96BF3

Structure / Form


Applications

Bioactivity
View all TMIGD2/CD28H Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TMIGD2/CD28H protein
Leu23-Gly150, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

15 kDa

SDS-PAGE

21-34 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TMIGD2/CD28H is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human
B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) that produces 50% of the optimal binding response is approximately 0.2-1 μg/mL.

Scientific Data Images for Recombinant Human TMIGD2/CD28H His-tag Protein, CF

Recombinant Human TMIGD2/CD28H His-tag Protein Bioactivity

Recombinant Human TMIGD2/CD28H His-tag Protein Bioactivity

When Recombinant Human TMIGD2/CD28H (Catalog # 9236-TR) is immobilized at 0.125 µg/mL, Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) binds with an ED50 of 0.2-1 µg/mL.

Formulation, Preparation, and Storage

9236-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Entrez Gene IDs

126259 (Human)

Gene Symbol

TMIGD2

UniProt

Q96BF3

Additional TMIGD2/CD28H Products

Product Documents for Recombinant Human TMIGD2/CD28H His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TMIGD2/CD28H His-tag Protein, CF

For research use only

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