Recombinant Human TRAIL R1/TNFRSF10A Fc Chimera Protein

A New rhTRAIL R1 is Now Available! ~2 fold better activity; CHO expressed; No his tag!
New Product 6949-DR.
(5 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 1-3 ng/mL in the presence of 6 ng/mL of Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC).
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived
    Human TRAIL R1
    (Ala109 - Asn239) &
    (Thr110 - Asn239)
    Accession # AAC51226
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala109 & Thr110
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    43 kDa (monomer)
  • SDS-PAGE
    46 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
347-DR
 
347-DR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R1/TNFRSF10A

Human TRAIL R1 (TNF-related apoptosis inducing ligand receptor 1), also called DR4 (death receptor 4), is a 50 ‑ 57 kDa, 468 amino acid (aa) type 1 transmembrane protein in the TNF R family, designated TNFRSF10A (1 ‑ 3). Of the five receptors for TRAIL (also called APO2 ligand) in humans, TRAIL R1/DR4 and TRAIL R2/DR5 are apoptosis-inducing and share 59% aa identity within the cytoplasmic death domain (aa 365 ‑ 448 of human TRAIL R1) (1 ‑ 3). Rodents do not express TRAIL R1/DR4. Human decoy receptors TRAIL R3 and TRAIL R4 have non-functional death domains but share ~60% aa identity with TRAIL R1 within extracellular TNFR domains (aa 107 ‑ 229), while the secreted TRAIL receptor osteoprotegerin shares only 18% aa identity with TRAIL R1. Trimeric TRAIL engagement of TRAIL R1 induces apoptosis by recruiting a cytosolic death-inducing signaling complex (DISC) that includes the adaptor FADD and caspases 8 and 10 (2 ‑ 6). TRAIL R1 translational modifications, such as O-glycosylation and palmitoylation, promote apoptosis by enhancing oligomerization and presence in lipid rafts, respectively (3 ‑ 6). Recombinant human TRAIL R1 neutralizes the ability of TRAIL to induce apoptosis (1). TRAIL R1 protein is expressed mainly in damaged, infected and malignant cells and can be repressed by hedgehog proteins in oncogenic cells (3, 7, 8). TRAIL R1 is also expressed on hemopoietic cells and influences activities such as monocyte migration (9). On senescent neutrophils, CXCL12/SDF-1 engagement of CXCR4 induces expression of all TRAIL R (7). TRAIL functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells (3, 10, 11). TRAIL R1 surface expression correlates more closely than that of TRAIL R2 with TRAIL induction of apoptosis on tumor cells such as melanoma and chronic lymphocytic and acute myeloid leukemias (6, 12, 13).

  • References:
    1. Pan, G. et al. (1997) Science 276:111.
    2. Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
    3. Yang, A. et al. (2010) Curr. Opin. Cell Biol. 22:837.
    4. Rossin, A. et al. (2009) Biohem. J. 419:185.
    5. Psahoulia, F.H. et al. (2007) Mol. Cancer Ther. 6:2591.
    6. Ries, C.R. et al. (2010) Cell Death Dis. 1:e83.
    7. Lum, J.J. et al. (2005) J. Immunol. 175:1232.
    8. Kurita, S. et al. (2010) Oncogene 29:4848.
    9. Wei, W. et al. (2010) Mol. Immunol. 47:2475.
    10. Sung, E-S. et al. (2009) Mol. Cancer Ther. 8:2276.
    11. Mom, C.H. et al. (2009) Clin. Cancer Res. 15:5584.
    12. MacFarlane, M. et al. (2005) Cell Death Differ. 12:773.
    13. Kurbanov, B.M. et al. (2005) J. Invest. Dermatol. 125:1010.
  • Long Name:
    TRAIL Receptor 1
  • Entrez Gene IDs:
    8797 (Human)
  • Alternate Names:
    APO2; CD261 antigen; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4 TRAIL receptor 1; DR4; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAILR1; TRAIL-R1; TRAILR-1; TRAILR1MGC9365; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily, member 10a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion.
    Authors: Peppa, Dimitra, Gill, Upkar S, Reynolds, Gary, Easom, Nicholas, Pallett, Laura J, Schurich, Anna, Micco, Lorenzo, Nebbia, Gaia, Singh, Harsimra, Adams, David H, Kennedy, Patrick, Maini, Mala K
    J Exp Med, 2013;210(1):99-114.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  2. Combination effect of equol and TRAIL against human cervical cancer cells.
    Authors: Kim E, Kim A
    Anticancer Res, 2013;33(3):903-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells.
    Authors: Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J
    J. Clin. Invest., 2012;122(7):2369-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Generation of new TRAIL mutants DR5-A and DR5-B with improved selectivity to death receptor 5.
    Authors: Gasparian ME, Chernyak BV, Dolgikh DA, Yagolovich AV, Popova EN, Sycheva AM, Moshkovskii SA, Kirpichnikov MP
    Apoptosis, 2009;14(6):778-87.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  5. Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway.
    Authors: He Q, Lee DI, Rong R, Yu M, Luo X, Klein M, El-Deiry WS, Huang Y, Hussain A, Sheikh MS
    Oncogene, 2002;21(17):2623-33.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

BA 375-TEC 9
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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF

BA 375-TEC/CF 9
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