Recombinant Human Transglutaminase 1/TGM1 Protein, CF

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7765-TG-050
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Recombinant Human Transglutaminase 1/TGM1 Protein, CF Summary

Purity
>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine. The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 1/TGM1 protein
Met2-Ala817, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Analysis
Inconclusive. Gly6, Ser7, Thr491, Ala571 identified. Poly His detected by Western blot.
Predicted Molecular Mass
89 kDa
SDS-PAGE
75-88 kDa, reducing conditions

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Background: Transglutaminase 1/TGM1

Transglutaminase 1, also known as TGM1, Transglutaminase K, keratinocyte transglutaminase, or epidermal TGase, is a member of the transglutaminase family. Transglutaminases catalyze the calcium-dependent formation of isopeptide bonds between lysine and glutamine residues (1). TGM1 plays an important role in formation of the cornified envelope of differentiating keratinocytes in the epidermis through its cross-linking of epidermal proteins (2). The majority of cases of lamellar ichthyosis result from mutations present within the TGM1 gene and the subsequent dysfunction of TGM1 (1, 3).

References
  1. Lorand, L. and R.M. Graham (2003) Nature Rev. Mol. Cell. Biol. 4:140.
  2. Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
  3. Farasat, S. et al. (2009) J. Med. Genet. 46:103.
Entrez Gene IDs
7051 (Human); 21816 (Mouse); 60335 (Rat)
Alternate Names
Epidermal TGase; ICR2; KTG; LI; LI1; LI1EC 2.3.2.13; protein-glutamine gamma-glutamyltransferase K; protein-glutamine-gamma-glutamyltransferase); TG(K); TGASE; TGase-1; TGK; TGKTGase K; TGM1; transglutaminase 1 (K polypeptide epidermal type I; Transglutaminase 1; Transglutaminase K; transglutaminase, keratinocyte; transglutaminase-1

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