Recombinant Human Transglutaminase 1/TGM1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Transglutaminase 1/TGM1 Protein, CF Summary

Product Specifications

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine. The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 1/TGM1 protein
Met2-Ala817, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Inconclusive. Gly6, Ser7, Thr491, Ala571 identified. Poly His detected by Western blot.
Predicted Molecular Mass
89 kDa
75-88 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: deionized water
  • Recombinant Human Transglutaminase 1/TGM1 (rhTGM1) (Catalog # 7765-TG)
  • Substrate: Z-Gln-Gly (Sigma, Catalog # C6154), 500 mM, pH 9.0 in deionized water
  • Hydroxylamine Hydrochloride (Sigma, Catalog # 159417), 1 M, pH 6.0 in deionized water
  • Stop solution: 0.37 M FeCl3 (Sigma, Catalog # 236489), 0.67 M HCl, 0.2 M Trichloroacetic acid
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Prepare Substrate Mixture containing 62.5 mM Substrate, 250 mM MES pH 6.0, 12.5 mM DTT, 12.5 mM CaCl2, and 125 mM Hydroxylamine Hydrochloride.
  2. Dilute rhTGM1 to 0.2 mg/mL in Assay Buffer.
  3. Mix 30 µL dilute rhTGM1 with 120 µL Substrate Mixture. Include an Enzyme Control containing 30 µL Assay Buffer with 120 µL Substrate Mixture.
  4. Incubate at 37 °C for 2 hours.
  5. Stop each reaction with 600 µL of Stop Solution. Mix well.
  6. Centrifuge at top speed for 2 minutes in a microcentrifuge.
  7. Load 200 µL of the supernatant into a 96 well clear plate.
  8. Read plate at 525 nm (absorbance) in endpoint mode.
  9. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Enzyme Control
     **Derived using calibration standard L-glutamic acid gamma -monohydroxamate (Sigma, Catalog # G2253).

Per Well:
  • rhTGM1: 1.6 µg
  • Substrate: 10 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Transglutaminase 1/TGM1

Transglutaminase 1, also known as TGM1, Transglutaminase K, keratinocyte transglutaminase, or epidermal TGase, is a member of the transglutaminase family. Transglutaminases catalyze the calcium-dependent formation of isopeptide bonds between lysine and glutamine residues (1). TGM1 plays an important role in formation of the cornified envelope of differentiating keratinocytes in the epidermis through its cross-linking of epidermal proteins (2). The majority of cases of lamellar ichthyosis result from mutations present within the TGM1 gene and the subsequent dysfunction of TGM1 (1, 3).

  1. Lorand, L. and R.M. Graham (2003) Nature Rev. Mol. Cell. Biol. 4:140.
  2. Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
  3. Farasat, S. et al. (2009) J. Med. Genet. 46:103.
Entrez Gene IDs
7051 (Human); 21816 (Mouse); 60335 (Rat)
Alternate Names
Epidermal TGase; ICR2; KTG; LI; LI1; LI1EC; protein-glutamine gamma-glutamyltransferase K; protein-glutamine-gamma-glutamyltransferase); TG(K); TGASE; TGase-1; TGK; TGKTGase K; TGM1; transglutaminase 1 (K polypeptide epidermal type I; Transglutaminase 1; Transglutaminase K; transglutaminase, keratinocyte; transglutaminase-1


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