Recombinant Human Transglutaminase 4/TGM4 Protein, CF

R&D Systems | Catalog # 5760-TG

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human Transglutaminase 4/TGM4 Protein (5760-TG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

AAC50516

Applications

Enzyme Activity
View all Transglutaminase 4/TGM4 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 4/TGM4 protein
Met2-Lys684, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

78 kDa

SDS-PAGE

65-70 kDa, reducing conditions

Activity

Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine.
The specific activity is >50 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

5760-TG
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Transglutaminase 4/TGM4

Transglutaminase 4, also known as TGM4, TG4, hTGP, or the prostate-specific transglutaminase, is a member of the transglutaminase family that is exclusively expressed and secreted from the prostate (1, 2). Transglutaminases catalyze the calcium-dependent formation of isopeptide bonds between lysine and glutamine residues (3). In rodents, TGM4 is responsible for the formation of the copulatory plug and sperm antigenicity, but the function in humans is largely unknown (4). The expression of TGM4 is primarily regulated by retinoic acid and secondarily modulated by androgens (5).

References

  1. Dubbink, H.J. et al. (1996) Biochem. J. 315:901.
  2. Dubbink, H.J. et al. (1999) Lab. Invest. 79:141.
  3. Lorand, L. and Graham, R.M. (2003) Nature Rev. Mol. Cell. Biol. 4:140.
  4. Williams-Ashman, H.G. et al. (1972) Proc. Natl. Acad. Sci. USA 69:2322.
  5. Rivera-Gonzalez, G.C. et al. (2012) Nucleic Acids Res. 40:4825.

Alternate Names

Fibrinoligase, TGM4, TGP

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Gene Symbol

TGM4

UniProt

AAC50516

Additional Transglutaminase 4/TGM4 Products

Product Documents for Recombinant Human Transglutaminase 4/TGM4 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Transglutaminase 4/TGM4 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Extracellular Matrix Molecules

Customer Reviews for Recombinant Human Transglutaminase 4/TGM4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Transglutaminase 4/TGM4 Protein, CF and earn rewards!

Have you used Recombinant Human Transglutaminase 4/TGM4 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human Transglutaminase 4/TGM4 Protein, CF (5760-TG):

Materials
  • Assay Buffer: deionized water
  • Recombinant Human Transglutaminase 4/TGM4 (rhTGM4) (Catalog # 5760-TG)
  • Substrate: Z-Gln-Gly (Sigma, Catalog # C6154), 500 mM, pH 9.0 in deionized water
  • Hydroxylamine Hydrochloride (Sigma, Catalog # 159417), 1 M, pH 6.0 in deionized water
  • Stop solution: 0.37 M FeCl3 (Sigma, Catalog # 236489), 0.67 M HCl, 0.2 M Trichloroacetic acid
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Prepare Substrate Mixture containing 62.5 mM substrate, 250 mM sodium acetate pH 5.0, 12.5 mM DTT, 200 mM CaCl2, 125 mM hydroxylamine hydrochloride, and 627 mM NaCl in deionized water.
  2. Dilute rhTGM4 to 0.2 mg/mL in Assay Buffer.
  3. Mix 30 µL dilute rhTGM4 with 120 µL Substrate Mixture. Include an enzyme blank containing 30 µL Assay Buffer with 120 µL Substrate Mixture.
  4. Incubate at 37 °C for 2 hours.
  5. Stop each reaction with 600 µL of Stop Solution. Mix well.
  6. Centrifuge at top speed for 2 minutes in a microcentrifuge.
  7. Load 200 µL of the supernatant into a 96 well clear plate.
  8. Read plate at 525 nm (absorbance) in endpoint mode.
  9. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard L-glutamic acid gamma -monohydroxamate (Sigma, Catalog # G2253).

Per Well:
  • rhTGM4: 1.6 µg
  • Substrate: 10 mM
  • Hydroxylamine: 20 mM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes