Recombinant Human TREM-1 Fc Chimera Protein, CF

R&D Systems | Catalog # 1278-TR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human TREM-1 Fc Chimera Protein (1278-TR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9NP99

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TREM-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TREM-1 protein
Human TREM-1
(Ala21 - Arg200)
Accession # Q9NP99		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

47 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to block anti-TREM-1-induced TNF-alpha secretion by human peripheral blood mononuclear cells (PBMC).
At 1 µg/mL, Recombinant Human TREM‑1 Fc Chimera is able to block > 70% of Goat Anti-Human TREM-1 (20 µg/mL, 200 µL/well) (Catalog # AF1278) induced TNF-alpha released from 1 x 105 cells.

Formulation, Preparation, and Storage

1278-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1 and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS‑induced shock. Blockade of TREM-1 with a TREM‑1/Fc fusion protein protected mice against LPS‑induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1 - 3).

References

  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9NP99

Additional TREM-1 Products

Product Documents for Recombinant Human TREM-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TREM-1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human TREM-1 Fc Chimera Protein, CF

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