Human TREM-1 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM‑1 and rmTREM‑2b is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
  Met1-Arg200
  Accession # Q9NP99
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 EU per 1 μg of the antibody by the LAL method.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Flow Cytometry
  2.5 µg/10⁶ cells
  Human whole blood monocytes and neutrophils
• Agonist Activity
  Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells. Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991.
  The ED₅₀ for this effect is typically 2 - 6 μg/mL.
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Human TREM-1 Sandwich Immunoassay
  Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 
Human TREM‑1 Antibody (Catalog # AF1278 )
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 
Human TREM‑1 Biotinylated Antibody (Catalog # BAF1278 )
ELISA Standard
 
Recombinant Human TREM-1 Fc Chimera Protein, CF (Catalog # 1278-TR )

Western Blot click image to view larger

Detection of Human TREM‑1 by Western Blot. Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

• Long Name:
  Triggering Receptor Expressed on Myeloid Cells 1

• Entrez Gene IDs:
  54210 (Human); 58217 (Mouse)

• Alternate Names:
  CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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