Recombinant Human TSLP Protein

Formulations:
Catalog # Availability Size / Price Qty
1398-TS-010
Recombinant Human TSLP Protein Bioactivity
2 Images
Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human TSLP Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. Reche, P.A. et al. (2001) J. Immunol. 167:336. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived human TSLP protein
Tyr29-Gln159, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
15 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1398-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1398-TS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human TSLP (Catalog # 1398-TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50for this effect is 0.05-0.3 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

References
  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  5. Quentmeier, H. et al. (2001) Leukemia 15:1286.
Long Name
Thymic Stromal Lymphopoietin
Entrez Gene IDs
85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names
thymic stromal lymphopoietin; TSLP

Citations for Recombinant Human TSLP Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
    Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells
    Authors: T Hochdörfer, C Winkler, K Pardali, J Mjösberg
    Eur. J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion
    Authors: HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li
    Mol Med Rep, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Application of a phenotypic drug discovery strategy to identify biological and chemical starting points for inhibition of TSLP production in lung epithelial cells
    Authors: A Orellana, V García-Gon, R López, S Pascual-Gu, E Lozoya, J Díaz, D Casals, A Barrena, S Paris, M Andrés, V Segarra, D Vilella, R Malhotra, P Eastwood, A Planagumà, M Miralpeix, A Nueda
    PLoS ONE, 2018;13(1):e0189247.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells
    Authors: A Camelo, G Rosignoli, Y Ohne, RA Stewart, C Overed-Say, MA Sleeman, RD May
    Blood Adv, 2017;1(10):577-589.
    Species: Human
    Sample Types: Whole Cells
  6. Whole-Genome Sequencing of a Family with Hereditary Pulmonary Alveolar Proteinosis Identifies a Rare Structural Variant Involving CSF2RA/CRLF2/IL3RA Gene Disruption
    Authors: CY Chiu, SC Su, WL Fan, SH Lai, MH Tsai, SH Chen, KS Wong, WH Chung
    Sci Rep, 2017;7(0):43469.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis
    Authors: Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng
    Biosci. Rep., 2017;37(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
    Authors: JT Schroeder, AP Bieneman
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps
    Authors: JA Poposki, AI Klingler, BK Tan, P Soroosh, H Banie, G Lewis, KE Hulse, WW Stevens, AT Peters, LC Grammer, RP Schleimer, KC Welch, SS Smith, DB Conley, JR Raviv, JG Karras, O Akbari, RC Kern, A Kato
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils
    Authors: B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie
    Oncol Lett, 2017;14(6):7483-7488.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Effector and regulatory dendritic cells display distinct patterns of miRNA expression
    Authors: V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Myeloid dendritic cells exhibit defects in activation and function in patients with multiple sclerosis
    Authors: Brigitte Wildemann
    J. Neuroimmunol., 2016;301(0):53-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Distinct roles of short and long thymic stromal lymphopoietin isoforms in house dust mite-induced asthmatic airway epithelial barrier disruption
    Sci Rep, 2016;6(0):39559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Abnormal thymic stromal lymphopoietin expression in the duodenal mucosa of patients with coeliac disease
    Authors: Gino R Corazza
    Gut, 2016;65(10):1670-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  16. TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis.
    Authors: Shin J, Kim S, Kim H, Noh J, Jin S, Park C, Lee W, Lee D, Lee J, Lee K
    J Invest Dermatol, 2016;136(2):507-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Thymic stromal chemokine TSLP acts through Th2 cytokine production to induce cutaneous T cell lymphoma
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Abnormal thymic stromal lymphopoietin expression in the duodenal mucosa of patients with coeliac disease
    Gut, 2016;65(10):1670-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISpot Development
  20. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015;212(5):619-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The Additive Inflammatory In Vivo and In Vitro Effects of IL-7 and TSLP in Arthritis Underscore the Therapeutic Rationale for Dual Blockade.
    Authors: Hillen M, Hartgring S, Willis C, Radstake T, Hack C, Lafeber F, van Roon J
    PLoS ONE, 2015;10(6):e0130830.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Directional secretory response of double stranded RNA-induced thymic stromal lymphopoetin (TSLP) and CCL11/eotaxin-1 in human asthmatic airways.
    Authors: Nino G, Huseni S, Perez G, Pancham K, Mubeen H, Abbasi A, Wang J, Eng S, Colberg-Poley A, Pillai D, Rose M
    PLoS ONE, 2014;9(12):e115398.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. NADPH oxidase-independent formation of extracellular DNA traps by basophils.
    Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
    J Immunol, 2014;192(11):5314-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
    Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
    PLoS ONE, 2014;9(8):e105498.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Thymic stromal lymphopoietin links keratinocytes and dendritic cell-derived IL-23 in patients with psoriasis.
    Authors: Volpe E, Pattarini L, Martinez-Cingolani C, Meller S, Donnadieu M, Bogiatzi S, Fernandez M, Touzot M, Bichet J, Reyal F, Paronetto M, Chiricozzi A, Chimenti S, Nasorri F, Cavani A, Kislat A, Homey B, Soumelis V
    J Allergy Clin Immunol, 2014;134(2):373-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa.
    Authors: Melum G, Farkas L, Scheel C, Van Dieren B, Gran E, Liu Y, Johansen F, Jahnsen F, Baekkevold E
    J Allergy Clin Immunol, 2014;134(3):613-621.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Macrophages participate in IL-17-mediated inflammation.
    Authors: Barin JG, Baldeviano GC, Talor MV, Wu L, Ong S, Quader F, Chen P, Zheng D, Caturegli P, Rose NR, Cihakova D
    Eur. J. Immunol., 2012;42(3):726-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
    Authors: Hvid M, Vestergaard C, Kemp K
    J. Invest. Dermatol., 2011;131(1):150-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
    Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
    J. Allergy Clin. Immunol., 2010;125(4):844-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. TSLP and downstream molecules in experimental mouse allergic conjunctivitis.
    Authors: Zheng X, Ma P, De Paiva CS, Cunningham MA, Hwang CS, Pflugfelder SC, Li DQ
    Invest. Ophthalmol. Vis. Sci., 2010;51(6):3076-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. FcepsilonRI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells.
    Authors: Okayama Y, Okumura S, Sagara H, Yuki K, Sasaki T, Watanabe N, Fueki M, Sugiyama K, Takeda K, Fukuda T, Saito H, Ra C
    Eur. Respir. J., 2009;34(2):425-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.
    Authors: Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA
    Blood, 2008;112(9):3762-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008;20(10):1321-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
    Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
    J. Clin. Invest., 2007;117(12):3868-3878.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
    Authors: Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D
    J Immunol, 0;192(4):1502-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human TSLP Protein
By Anonymous on 12/06/2017
Application: In vitro bioactivity in cell culture