Recombinant Human TSLP Protein

(27 citations)
(1 Review)
  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. Reche, P.A. et al. (2001) J. Immunol. 167:336. The ED50 for this effect is 0.05-0.3 ng/mL.
  • Source
    E. coli-derived Tyr29-Gln159, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    15 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1398-TS
 
1398-TS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TSLP (Catalog # 1398‑TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50 for this effect is 0.05-0.3 ng/mL.
1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

  • References:
    1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
    2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
    4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
    5. Quentmeier, H. et al. (2001) Leukemia 15:1286.
  • Long Name:
    Thymic Stromal Lymphopoietin
  • Entrez Gene IDs:
    85480 (Human); 53603 (Mouse)
  • Alternate Names:
    thymic stromal lymphopoietin; TSLP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
    Authors: JT Schroeder, AP Bieneman
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Whole-Genome Sequencing of a Family with Hereditary Pulmonary Alveolar Proteinosis Identifies a Rare Structural Variant Involving CSF2RA/CRLF2/IL3RA Gene Disruption
    Authors: CY Chiu, SC Su, WL Fan, SH Lai, MH Tsai, SH Chen, KS Wong, WH Chung
    Sci Rep, 2017;7(0):43469.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis
    Authors: Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng
    Biosci. Rep., 2017;37(4):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Abnormal thymic stromal lymphopoietin expression in the duodenal mucosa of patients with coeliac disease
    Gut, 2016;65(10):1670-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Elispot Development
  5. Thymic stromal chemokine TSLP acts through Th2 cytokine production to induce cutaneous T cell lymphoma
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis.
    Authors: Shin J, Kim S, Kim H, Noh J, Jin S, Park C, Lee W, Lee D, Lee J, Lee K
    J Invest Dermatol, 2016;136(2):507-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Distinct roles of short and long thymic stromal lymphopoietin isoforms in house dust mite-induced asthmatic airway epithelial barrier disruption
    Sci Rep, 2016;6(0):39559.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015;212(5):619-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. The Additive Inflammatory In Vivo and In Vitro Effects of IL-7 and TSLP in Arthritis Underscore the Therapeutic Rationale for Dual Blockade.
    Authors: Hillen M, Hartgring S, Willis C, Radstake T, Hack C, Lafeber F, van Roon J
    PLoS ONE, 2015;10(6):e0130830.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
    Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
    PLoS ONE, 2014;9(8):e105498.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Thymic stromal lymphopoietin links keratinocytes and dendritic cell-derived IL-23 in patients with psoriasis.
    Authors: Volpe E, Pattarini L, Martinez-Cingolani C, Meller S, Donnadieu M, Bogiatzi S, Fernandez M, Touzot M, Bichet J, Reyal F, Paronetto M, Chiricozzi A, Chimenti S, Nasorri F, Cavani A, Kislat A, Homey B, Soumelis V
    J Allergy Clin Immunol, 2014;134(2):373-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. NADPH oxidase-independent formation of extracellular DNA traps by basophils.
    Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
    J Immunol, 2014;192(11):5314-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Directional secretory response of double stranded RNA-induced thymic stromal lymphopoetin (TSLP) and CCL11/eotaxin-1 in human asthmatic airways.
    Authors: Nino G, Huseni S, Perez G, Pancham K, Mubeen H, Abbasi A, Wang J, Eng S, Colberg-Poley A, Pillai D, Rose M
    PLoS ONE, 2014;9(12):e115398.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa.
    Authors: Melum G, Farkas L, Scheel C, Van Dieren B, Gran E, Liu Y, Johansen F, Jahnsen F, Baekkevold E
    J Allergy Clin Immunol, 2014;134(3):613-621.e7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Macrophages participate in IL-17-mediated inflammation.
    Authors: Barin JG, Baldeviano GC, Talor MV, Wu L, Ong S, Quader F, Chen P, Zheng D, Caturegli P, Rose NR, Cihakova D
    Eur. J. Immunol., 2012;42(3):726-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
    Authors: Hvid M, Vestergaard C, Kemp K
    J. Invest. Dermatol., 2011;131(1):150-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
    Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
    J. Allergy Clin. Immunol., 2010;125(4):844-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. TSLP and downstream molecules in experimental mouse allergic conjunctivitis.
    Authors: Zheng X, Ma P, De Paiva CS, Cunningham MA, Hwang CS, Pflugfelder SC, Li DQ
    Invest. Ophthalmol. Vis. Sci., 2010;51(6):3076-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. FcepsilonRI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells.
    Authors: Okayama Y, Okumura S, Sagara H, Yuki K, Sasaki T, Watanabe N, Fueki M, Sugiyama K, Takeda K, Fukuda T, Saito H, Ra C
    Eur. Respir. J., 2009;34(2):425-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008;20(10):1321-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.
    Authors: Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA
    Blood, 2008;112(9):3762-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
    Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
    J. Clin. Invest., 2007;117(12):3868-3878.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 27 Citations
Average Rating: 4 (Based on 1 review)

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