Recombinant Human UNC5B Protein Fc Chimera, CF

Catalog # Availability Size / Price Qty
8869-UN-050
Product Details
Citations (2)
FAQs
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Recombinant Human UNC5B Protein Fc Chimera, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human UNC5H2/UNC5B Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) that produces 50% optimal binding response is approximately 7-42 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human UNC5H2/UNC5B protein
Human UNC5H2/UNC5B
(Gly27-Tyr377)
Accession # Q8IZJ1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8869-UN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: UNC5H2/UNC5B

Netrin Receptor UNC5B is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. UNC5B is a type I transmembrane protein that contains two Ig-like and two thrombospondin domains in its extracellular domain (ECD), and a ZU5 domain, a DCC-binding domain, and a death domain in its intracellular region (1, 2). It is widely expressed in various embryonic and adult tissues including neural, hematopoietic, and immune tissues (1, 3, 4). Human UNC5B has a predicted molecular weight of approximately 104 kDa. Its ECD shares approximately 92% amino acid sequence identity within shared regions in the mouse and rat orthologs, which are termed UNC5H2. UNC5B regulates axon guidance by facilitating Netrin-1-mediated axon repulsion of neuronal growth cones (1). It complexes with the attractive Netrin receptor DCC and switches its response to Netrin-1 from attraction to repulsion (5). UNC5B can also induce growth cone collapse via its associations with the RGM-A receptor Neogenin and the soluble ECD of FLRT3 (2, 6). Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation (7-10). UNC5B also functions as a dependence receptor. In the absence of Netrin-1, UNC5B mediates p53-dependent apoptosis via activation of DAPK (11, 12). UNC5B is believed to play a role in tumorgenesis. It has been shown to inhibit tumor cell growth and invasion, and its expression appears to be down-regulated in multiple cancers including colorectal, breast, ovary, uterus, stomach, lung, and kidney (13, 14). Additionally, UNC5B is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury (15, 16).

References
  1. Leonardo, E.D. et al. (1997) Nature 386:833.
  2. Hata, K. et al. (2009) J. Cell Biol. 184:737.
  3. Engelkamp, D. (2002) Mech. Dev. 118:191.
  4. Manitt, C. et al. (2004) J. Neurosci. Res. 77:690.
  5. Hong, K. et al. (1999) Cell 97:927.
  6. Yamagishi, S. et al. (2011) EMBO J. 30:2920.
  7. Lu, X. et al. (2004) Nature 432:179.
  8. Ly, N.P. et al. (2005) Proc. Natl. Acad. Sci. USA 102:14729.
  9. Larrivée, B. et al. (2007) Genes Dev. 21:2433.
  10. Mediero, A. et al. (2015) J. Bone Miner. Res. 30:837.
  11. Tanikawa, C. et al. (2003) Nat. Cell Biol. 5:216.
  12. Llambi, F. et al. (2005) EMBO J. 24:1192.
  13. Thiébault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
  14. Arakawa, H. (2004) Nat. Rev. Cancer 4:978.
  15. Oksala, N. et al. (2013) Circ. Cardiovasc. Genet. 6:579.
  16. Ranganathan, P. et al. (2014) J. Am. Soc. Nephrol. 25:239.
Long Name
Netrin Receptor UNC5B
Entrez Gene IDs
219699 (Human); 60630 (Rat)
Alternate Names
p53RDL1; p53-regulated receptor for death and life protein 1; Protein unc-5 homolog 2; Protein unc-5 homolog B; transmembrane receptor Unc5H2; unc5 (C.elegans homolog) b; unc-5 homolog 2; unc-5 homolog B (C. elegans); UNC5B; UNC5H2; UNC5H2netrin receptor UNC5B

Citations for Recombinant Human UNC5B Protein Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling
    Authors: I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota
    Development, 2017;0(0):.
    Applications: Bioassay

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