Recombinant Human VEGF 189 (aa 27-215) Protein

Formulations:
Catalog # Availability Size / Price Qty
8147-VE-025
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human VEGF 189 (aa 27-215) Protein Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 4-40 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human VEGF protein
Ala27-Arg215
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
22 kDa (monomer)
SDS-PAGE
22-30 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8147-VE

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8147-VE/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

VEGF189 (Vascular Endothelial Growth Factor-A of 189 amino acids) is a 50-52 kDa homodimeric glycoprotein member of the PDGF/VEGF family of molecules (1, 2). A large number of human VEGF isoforms are generated by alternative splicing, including VEGF189 which lacks amino acids (aa) 166-182 of the full length protein (VEGF206) (3-5). Mature human VEGF189 shares 88% aa sequence identity with mouse VEGF188. VEGF189 binds to heparan sulfate both on the cell surface and in the extracellular matrix (ECM) where it retains bioactivity (6, 7). It can undergo proteolytic cleavage from the ECM (at Arg136-Ala137), creating a soluble fragment with increased bioactivity (6, 7). VEGF189 is widely expressed including by vascular smooth muscle cells (3), chondrocytes (8), fibroblasts (9), neuronal and glial cells (10), and neutrophils (11). VEGF189 interacts with various receptors including VEGF R1, VEGF R2, Neuropilin-1, and Integrins aVb3, aVb5, a3b1, and a5b1 (6, 12-14). VEGF189 exerts complex effects in angiogenesis. It is proapoptotic towards quiescent vascular endothelium but is proangiogenic towards endothelium that has committed to the angiogenic process (6, 14). In particular, venous endothelial cells are highly responsive towards VEGF189, while the responsiveness of arterial endothelium is primarily restricted to the regulation of migration (7). In addition to its vascular effects,  immobilized VEGF189 serves as an anchor for cells expressing Integrin avb3, and it acts as both a chemotactic and chemokinetic factor for migrating neutrophils (11, 13, 14).

References
  1. Woolard, J. et al. (2009) Microcirculation 16:572.
  2. Ferrara, N. et al. (2003) Nat. Med. 9:669.
  3. Tischer, E. et al. (1991) J. Biol. Chem. 266:11947.
  4. Leung, D.W. et al. (1989) Science 246:1306.
  5. Keck, P.J. et al. (1989) Science 246:1309.
  6. Plouet, J. et al. (1997) J. Biol. Chem. 272:13390.
  7. Herve, M-A. et al. (2005) Exp. Cell Res. 309:24.
  8. Cramer, T. et al. (2004) Osteoarthritis Cartilage 12:433.
  9. Detmar, M. et al. (1997) J. Invest. Dermatol. 108:263.
  10. Tang, H. et al. (2013) Neurobiol. Aging 34:1412.
  11. Houck, K.A. et al. (1992) J. Biol. Chem. 267:26031.
  12. Herve, M-A. et al. (2008) Am. J. Pathol. 172:167.
  13. Hutchings, H. et al. (2003) FASEB J. 17:1520.
  14. Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.
Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Citation for Recombinant Human VEGF 189 (aa 27-215) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer
    Authors: A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    • Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  2. What is the recommended method for reconstitution of a lyophilized protein or antibody?

    • Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.  

       

  3. Are R&D Systems recombinant proteins and antibodies sterile?

    • Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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