Recombinant Human Versican Isoform V3 His-tag Protein, CF

Catalog # Availability Size / Price Qty
3054-VN-050
Recombinant Human Versican Isoform V3 His-tag Protein, CF Binding Activity
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Recombinant Human Versican Isoform V3 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Source
Chinese Hamster Ovary cell line, CHO-derived human Versican protein
Leu21-Tyr646, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu21
Predicted Molecular Mass
71 kDa
SDS-PAGE
78-89 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3054-VN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human Versican Isoform V3 (Catalog # 3054-VN) is coated at 0.25 μg/mL, 100 μL/well, Biotinylated Hyalurinan binds with an ED50 of 0.25-1.5 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Versican Isoform V3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 78-89 kDa and 67-80 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Versican

Versican (versatile proteoglycan; also known as PG-M, CSPG2, ERVR, GHAP, WGN, WGN1) is a 500-600 kDa, secreted member of the large aggregating chondroitin sulfate proteoglycan family, which includes aggrecan, neurocan, and brevican (1-3). The canonical sequence of human Versican (Isoform V0) is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region, two large, alternately spliced GAG attachment domains (GAG alpha and GAG beta), two EGF-like domains, a selectin-like region, and a complement regulatory protein-like domain (2). Human Versican has multiple alternative splice variants that include variations mainly in the GAG regions. This product is the Isoform V3 which includes one mutation at aa 348 (Pro to Arg) and lacks of the entire GAG domains (aa 349-3089) compared to Isoform V0. Versican is known to associate with hyaluronan (4), tenascin (5-7), fibronectin (8), fibulin (9), and CD44 (10) in the extracellular matrix that may contribute to the malignant properties of tumor cells (11). It has an anti-adhesive effect on cells, facilitating tumor migration and invasion (12). The multiple alternative splice variants of human Versican could affect cell-cell and cell-matrix adhesion (13-15), migration (16), proliferation, apoptosis (17), and mesenchymal-epithelial transition (18). The Versican gene is expressed in several different tissues, and it is over-expressed in several cancer types, including prostate (19), brain (20), ovary (21), breast (22) and melanoma (11).

References
  1. Shinomura, T. et al. (1993) J. Biol. Chem. 268:14461.
  2. Zimmermann, D. R. and E.Ruoslahti (1989) EMBO J. 8:2975.
  3. Margolis, R. U. and R. K. Margolis (1994) Methods Enzymol. 245:105.
  4. Lebaron, R. G. et al. (1992) J. Biol. Chem. 267:10003.
  5. Perides, G. et al. (1993) Anat. Embryol. (Berl.) 188:467.
  6. Aspberg, A. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:10590.
  7. Aspberg, A. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:10116.
  8. Braunewell, K. H. et al. (1995) Eur. J. Neurosci. 7:805.
  9. Olin, A. I. et al. (2001) J. Biol. Chem. 276:1253.
  10. Kawashima, H. et al. (2000) J. Biol. Chem. 275:35448.
  11. Touab, M. et al. (2002) Am. J. Pathol. 160:549.
  12. Yamagata, M. et al. (1989) J. Biol. Chem. 264:8012.
  13. Wu, Y. et al. (2002) J. Biol. Chem. 277:12294.
  14. Zheng, P. S. et al. (2004) J. Cell Sci. 117:5887.
  15. Lemire, J. M. et al. (2002) J. Cell Physiol. 190:38.
  16. Mjaatvedt, C.H. et al. (1998) Dev. Biol. 202:56.
  17. Sheng, W. et al. (2005) Mol. Biol. Cell. 16:1330.
  18. Sheng, W. et al. (2006) Mol. Biol. Cell. 17:2009.
  19. Ricciardelli, C. et al. (1998) Clin. Cancer Res. 4:963.
  20. Paulus, W. et al. (1996) J. Neuropathol. Exp. Neurol. 55:528.
  21. Voutilainen, K. et al. (2003) Int. J. Cancer 107:359.
  22. Nara, Y. et al. (1997) Histochem. J. 29:21.
Entrez Gene IDs
1462 (Human)
Alternate Names
Chondroitin sulfate proteoglycan 2WGN; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2DKFZp686K06110; ERVR; GHAP; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PG-MWGN1; VCAN; versican core protein; versican proteoglycan; Versican

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