Recombinant Human VSIG3 Fc Chimera Protein, CF

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Recombinant Human VSIG3 Fc Chimera Protein Bioactivity
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Product Details
Citations (2)
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Reviews (1)

Recombinant Human VSIG3 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
Mouse myeloma cell line, NS0-derived human VSIG3 protein
Human VSIG3
Accession # BAC07546
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
56-69 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human VSIG3 Fc Chimera Protein Bioactivity View Larger

Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 1-6 μg/mL.

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Background: VSIG3

VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).

  1. Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
  2. Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
  3. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
  4. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
  5. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
  6. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
  7. Watanabe, T. et al. (2005) Cancer Sci. 96:498.
  8. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
Long Name
V-Set and Immunoglobulin Domain Containing 3
Entrez Gene IDs
152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)
Alternate Names
Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein

Citations for Recombinant Human VSIG3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation
    Authors: N Mehta, S Maddineni, II Mathews, R Andres Par, PS Huang, JR Cochran
    Cell Rep, 2019;28(10):2509-2516.e5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Human VSIG3 Fc Chimera Protein, CF
By Sarah Garrett on 05/22/2018
Application: Binding assay/Protein-protein interaction

0.5 ug VSIG3-hIgG1 (IGSF11) or hIgG1 were added to cells expressing hVISTA or a negative control. Binding was assessed using an anti-human 488 secondary antibody.