Recombinant Human VSIG3 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human VSIG3
    (Leu23-Gly245)
    Accession # BAC07546
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu23
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    50 kDa
  • SDS-PAGE
    56-69 kDa, reducing conditions
9229-VS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS)inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. TheED50 for this effect is 1-6 μg/mL.
Background: VSIG3
VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).
  • References:
    1. Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
    2. Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
    3. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
    4. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
    5. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
    6. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
    7. Watanabe, T. et al. (2005) Cancer Sci. 96:498.
    8. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
  • Long Name:
    V-Set and Immunoglobulin Domain Containing 3
  • Entrez Gene IDs:
    152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)
  • Alternate Names:
    Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein
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