Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human Wnt-1/sFRP-1 Complex Bioactivity.
1 Image
Product Details

Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human Wnt-1 protein
Human Wnt-1
Accession # P04628.1
Human sFRP-1
Accession # AAB70793.1
N-terminus C-terminus
N-terminal Sequence
Ala28 (Wnt-1) & Ser32 (sFRP-1)
Structure / Form
Non-covalent complex
Predicted Molecular Mass
38 kDa (Wnt-1) & 33 kDa (sFRP-1)
34-58 kDa, under reducing conditions.

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity View Larger

Recombinant Human Wnt-1/sFRP-1 Complex (Catalog # 11160-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Wnt-1

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Human Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Human Wnt -1 shares a 99.7% aa sequence identity with mouse Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active human Wnt-1 protein in a complex with human sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

  1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
  3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
  4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  5. Korinek, V. et al. (1997) Science 275:1784.
  6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Long Name
Wingless-type MMTV Integration Site Family, Member 1
Entrez Gene IDs
7471 (Human); 22408 (Mouse)
Alternate Names
Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF and earn rewards!

Have you used Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review