Recombinant MERS-CoV Spike RBD His-tag Protein, CF

HEK293 Expressed
Catalog # Availability Size / Price Qty
10636-CV-100
Recombinant MERS-CoV Spike RBD His-tag Protein Binding Activity.
2 Images
Product Details
FAQs
Reviews

Recombinant MERS-CoV Spike RBD His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).
Source
Human embryonic kidney cell, HEK293-derived mers-cov Spike RBD protein
Glu367-Tyr606, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu367 & Lys369
Predicted Molecular Mass
27 kDa
SDS-PAGE
34-41 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10636-CV

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Recombinant MERS-CoV Spike RBD His-tag Protein Binding Activity. View Larger

Recombinant MERS-CoV Spike RBD His-tag (Catalog # 10636-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.

SDS-PAGE Recombinant MERS-CoV Spike RBD His-tag Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant MERS-CoV Spike RBD His-tag (Catalog # 10636-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-41 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Spike RBD

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), was first reported in Saudi Arabia in 2012 as a novel coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes MERS-CoV, HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Located within the S1 subunit is the receptor binding domain (RBD). The RBD is responsible for the binding of MERS-CoV to dipeptidyl peptidase IV (DPP4, also known as human CD26) (4). The RBD of MERS-CoV shares 24% and 21% amino acid sequence (aa) identity with SARS-CoV RBD and SARS-Cov2 RBD, respectively. The low aa sequence identity is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). The S1 subunit, especially the RBD region, of MERS-CoV was commonly targeted for vaccinations or antiviral therapies (5-7).

References
  1. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  2. Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  6. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  7. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.
Long Name
Spike Receptor Binding Domain
Entrez Gene IDs
3200426 (HCoV-HKU1); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names
2019-nCoV Peplomer protein; 2019-nCoV S Protein; 2019-nCoV Spike; COVID-19 Spike; E2; Human coronavirus spike glycoprotein; Peplomer protein; S glycoprotein; S protein; SARS-COV-2 E2; SARS-COV-2 Peplomer protein; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; SARS-CoV-2; Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein; Spike glycoprotein; Spike RBD; Spike S1 RBD; Spike S1; Spike; surface glycoprotein

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant MERS-CoV Spike RBD His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant MERS-CoV Spike RBD His-tag Protein, CF and earn rewards!

Have you used Recombinant MERS-CoV Spike RBD His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review