Recombinant MERS-CoV Spike RBD His-tag Protein, CF

R&D Systems | Catalog # 10636-CV

HEK293 Expressed
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant MERS-CoV Spike RBD His-tag Protein (10636-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

YP_007188579.1

Applications

Bioactivity
View all Spike RBD Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mers-cov Spike RBD protein
Glu367-Tyr606, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu367 & Lys369

Predicted Molecular Mass

27 kDa

SDS-PAGE

34-41 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).

Scientific Data Images for Recombinant MERS-CoV Spike RBD His-tag Protein, CF

Recombinant MERS-CoV Spike RBD His-tag Protein Binding Activity.

Recombinant MERS-CoV Spike RBD His-tag (Catalog # 10636-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.

Recombinant MERS-CoV Spike RBD His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant MERS-CoV Spike RBD His-tag (Catalog # 10636-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-41 kDa.

Formulation, Preparation, and Storage

10636-CV
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Spike RBD

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), was first reported in Saudi Arabia in 2012 as a novel coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes MERS-CoV, HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Located within the S1 subunit is the receptor binding domain (RBD). The RBD is responsible for the binding of MERS-CoV to dipeptidyl peptidase IV (DPP4, also known as human CD26) (4). The RBD of MERS-CoV shares 24% and 21% amino acid sequence (aa) identity with SARS-CoV RBD and SARS-Cov2 RBD, respectively. The low aa sequence identity is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). The S1 subunit, especially the RBD region, of MERS-CoV was commonly targeted for vaccinations or antiviral therapies (5-7).

References

  1. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  2. Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  6. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  7. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_007188579.1

Additional Spike RBD Products

Product Documents for Recombinant MERS-CoV Spike RBD His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant MERS-CoV Spike RBD His-tag Protein, CF

For research use only

Related Research Areas

Infectious Diseases

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