Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF

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Graph showing bioactivity of MERS Spike S1 Domain protein binding to Human DPPIV / CD26
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Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.

Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).

Chinese Hamster Ovary cell line, CHO-derived mers-cov Spike S1 Subunit protein
MERS-CoV Spike S1 Subunit
Accession # K9N5Q8.1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107 kDa
115-135 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing bioactivity of MERS Spike S1 Domain protein binding to Human DPPIV / CD26 View Larger

Recombinant MERS-CoV Spike S1 Subunit Fc Chimera (Catalog # 10606-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.

SDS-PAGE SDS-PAGE gel of purified recombinant MERS Spike S1 Domain protein, Fc chimera View Larger

2 μg/lane of Recombinant MERS-CoV Spike S1 Subunit Fc Chimera (Catalog # 10606-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 220-260 kDa, respectively.

Flow Cytometry Flow cytometry scatter plot shows MERS Spike S1 Domain protein binds to CD26 / DPPIV expressing HEK293 cells View Larger

In a functional flow cytometry test, (A) Recombinant MERS-CoV Spike S1 Subunit Fc chimera Protein (Catalog # 10606-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD26/DPPIV and EGFP. Ligand binding was detected by staining cells with APC-conjugated anti-Human IgG Fc Monoclonal Antibody (FAB110A), which does not stain the cells in the absence of recombinant protein (B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Spike S1 Subunit

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1,2). Other well-known human coronaviruses include several viruses that cause relatively mild respiratory disease, plus two viruses that caused the Severe Acute Respiratory Syndrome (SARS-CoV) and the global pandemic Covid-19 (SARS-CoV2). MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Based on amino acid (aa) sequence homology, the MERS-CoV S1 subunit shares 23% and 22% identity with SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence homology is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage ACE2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4 (DPP4; also known as CD26) as its functional receptor (4). Based on structural biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is located in the C-terminal region of S1 subunit and consists of a core subdomain and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region, was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).

  1. Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
  2. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Lu, G. et al. (2013) Nature 500:227.
  6. Wang, N. et al. (2013) Cell. Res. 23:986.
  7. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  8. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  9. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.
Long Name
Spike Protein, S1 Subunit
Alternate Names
SARS-CoV-2; Spike S1 Subunit


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Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF
By Anonymous on 01/17/2021
Application: Binding assay/Protein-protein interaction