Human IgG Fc APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110A
FAB110A-100
Human IgG Fc APC-conjugated Antibody in Data
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human IgG Fc APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IgG<SUB>1</SUB>Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. View Larger

Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

Citations for Human IgG Fc APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
    Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
    JCI Insight, 2019;4(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
    Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
    PLoS ONE, 2017;12(3):e0174103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human IgG Fc APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human IgG Fc APC-conjugated Antibody and earn rewards!

Have you used Human IgG Fc APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review