Recombinant Mouse ASGR2 Fc Chimera Protein, CF Summary
Accession # P24721.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Mouse ASGR2 Fc Chimera (10447-AS) binds human plasma von Willebrand Factor with an ED50 of 0.05-0.6 μg/mL.
2 μg/lane of Recombinant Mouse ASGR2 Fc Chimera (Catalog # 10447-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 120-160 kDa, respectively.
Asialoglycoprotein receptor 2 (ASGR2), also known as hepatic lectin 2 (HL-2), is an approximately 38 kDa member of the C-type lectin receptor family (1). ASGR2 is a single-pass type II transmembrane protein that plays a role in the endocytosis of desialylated glycoproteins, hepatic thrombopoietin production, and gastrointestinal malignancies (2, 3). ASGR2 is closely related to ASGR1 which is about 2 kDa smaller and is encoded by distinct but closely linked genes. Variations in ASGR2 structure due to alternative splicing have been reported (4-6). Mature ASGR2 consists of a cytoplasmic domain, transmembrane segment and an extracellular domain (ECD). Within the ECD, mouse ASGR2 shares 67% and 83% aa sequence identity with human and rat ASGR2, respectively. Both ASGR2 and ASGR1 compose the asialoglycoprotein receptor (ASGPR) which mediates removal of potentially hazardous glycoconjugates from blood in health and disease (7). ASGPR, also known as the Ashwell receptor, can modulate von Willebrand factor (vWF) and platelet homeostasis in part via clearance of platelets that are first desialylated during sepsis caused by pathogens including S. pneumoniae. The ASGR1 chain of the Ashwell receptor participates in plasma vWF clearance independently of sialylation and sepsis, ASGR2 colocalization with plasma vWF was increased in ASGR1 deficient mice (8). In addition, ASGR2 expression was shown to be associated with malignant phenotypes in gastric cancer and may represent a specific biomarker for recurrence of some gastric cancers (9).
- Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
- Grozovsky, R. et al. (2015) Nat Med. 1:47.
- Tanaka, H. et al. (2018) Mol. Cancer Res. 16:1420.
- Drickamer, K. et al. (1984) J. Biol. Chem. 259:770.
- Halberg, D.F. et al. (1987) J. Biol. Chem. 262:9828.
- Paietta, E. et al. (1992) J. Biol. Chem. 267:11078.
- Harris R.L. et al. (2012) Mol Biol Int. 2012:283974.
- Grewal, P. et al. (2008) Nature Medicine. 14:6.
- Tanaka H. et al. (2018) Mol Cancer Res. 16:1420.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse ASGR2 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse ASGR2 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse ASGR2 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image