Recombinant Mouse ASIP/Agouti Protein, CF

Catalog # Availability Size / Price Qty
9619-AG-050
Recombinant Mouse ASIP/Agouti Protein, CF Data
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Recombinant Mouse ASIP/Agouti Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse ASIP protein
His23-Cys131
Accession #
N-terminal Sequence
Analysis
His23
Predicted Molecular Mass
12 kDa
SDS-PAGE
19-24 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9619-AG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

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Recombinant Mouse ASIP/Agouti (Catalog # 9619-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50for this effect is 0.8-4 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASIP

Agouti, also known as Agouti Signaling Protein (ASIP), is a 131 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature mouse Agouti shares 78% and 94% amino acid sequence identity with human and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Studies of human Agouti have shown that it is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). In humans and mice, Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).

References
  1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
  2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
  3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
  4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
  5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
  6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
  7. Lu, D. et al. (1994) Nature 371:799.
  8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
  9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.
Long Name
Agouti Signaling Protein
Entrez Gene IDs
434 (Human); 50518 (Mouse); 24152 (Rat)
Alternate Names
agouti (mouse)-signaling protein; agouti signaling protein; agouti signaling protein, nonagouti homolog (mouse); agouti signaling protein, nonagouti homolog; Agouti switch protein; agouti-signaling protein; AGSW; AGTI; AGTIL; AGTILMGC126092; ASIP; ASPAGSW; MGC126093; nonagouti homolog; SHEP9

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