Recombinant Mouse ASIP/Agouti Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse ASIP/Agouti (Catalog # 9619-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50for this effect is 0.8-4 μg/mL.
Agouti, also known as Agouti Signaling Protein (ASIP), is a 131 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature mouse Agouti shares 78% and 94% amino acid sequence identity with human and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Studies of human Agouti have shown that it is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). In humans and mice, Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).
- Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
- Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
- Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
- Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
- Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
- Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
- Lu, D. et al. (1994) Nature 371:799.
- Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
- Kiefer, L.L. et al. (1997) Biochemistry 36:2084.
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