Recombinant Mouse B7-H2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
8127-B7-050
8127-B7-01M
Recombinant Mouse B7-H2 His-tag Protein, CF Bioactivity
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Recombinant Mouse B7-H2 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse B7-H2 protein
Glu47-Lys279, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu47
Predicted Molecular Mass
27 kDa
SDS-PAGE
50-70 kDa, reducing conditions

Product Datasheets

8127-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Mouse B7-H2 His-tag Protein, CF Bioactivity View Larger

Recombinant Mouse B7-H2
co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 µg/mL.

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Background: B7-H2

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H2 consists of a 231 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 24 aa cytoplasmic domain (2). Within the ECD, mouse B7-H2 shares 50% and 71% aa sequence identity with human and rat B7-H2, respectively. Alternative splicing generates a long isoform that carries a 27 aa substitution for the C-terminal 2 residues. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (3-6). It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (3, 4, 8). Mouse and human B7-H2 exhibit cross-species binding to ICOS (5, 7). The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (TFH) and germinal centers (3-5, 9, 10). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (7). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (9, 11, 12). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (6, 13, 14). In contrast, its ligation of ICOS on TFH cells can increase the severity of autoimmune symptoms (10). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (15). In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (16).

References
  1. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  2. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  3. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  4. Wang, S. et al. (2000) Blood 96:2808.
  5. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
  6. Faget, J. et al. (2012) Cancer Res. 72:6130.
  7. Yao, S. et al. (2011) Immunity 32:729.
  8. Watanabe, M. et al. (2008) J. Immunol. 180:5222.
  9. Wong, S.-C. et al. (2003) Blood 102:1381.
  10. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
  11. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
  12. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
  13. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
  14. Akbari, O. et al. (2002) Nat. Med. 8:1024.
  15. Her, M. et al. (2009) Lupus 18:501.
  16. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
Long Name
B7 Homolog 2
Entrez Gene IDs
23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand

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