Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

(4 citations)   
  • Purity
    >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1‑0.5 ng/mL in the presence of goat anti‑mouse IgM µ chain.
  • Source
    Mouse myeloma cell line, NS0-derived Ala127-Leu309
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala127
  • Structure / Form
    Noncovalently-linked homotrimer
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    19-23 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8876-BF
 
8876-BF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein..
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP.
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) induces mouse B cell proliferation. The ED50 for this effect is 0.1‑0.5 ng/mL.The ED50 for the two competitors is 5 ng/mL, which is more than 25-fold weaker.
1 μg/lane of Recombinant Mouse BAFF/BLyS/TNFSF13B was resolved with SDS-PAGE under either reducing (R) or non-reducing (NR) conditions and visualized by silver staining, showing a single band at 20 kDa
Background: BAFF/BLyS/TNFSF13B
B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature mouse BAFF consists of a 47 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 241 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 127-309 of the ECD, mouse BAFF shares 72% aa sequence identity with human BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg126 and Ala127 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that over-express BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).
  • References:
    1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
    2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
    3. Moore, P.A. et al. (1999) Science 285:260.
    4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
    5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
    6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
    7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
    8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
    9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
    10. Thompson, J.S. et al. (2001) Science 293:2108.
    11. Schiemann, B. et al. (2001) Science 293:2111.
    12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
    13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
    14. Huard, B. et al. (2001) J. Immunol. 167:6225.
    15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
    16. Walters, S. et al. (2009) J. Immunol. 182:793.
    17. Chang, S.K. et al. (2006) Blood 108:2687.
  • Long Name:
    B cell Activating Factor
  • Entrez Gene IDs:
    10673 (Human); 24099 (Mouse); 498666 (Rat)
  • Alternate Names:
    ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Gsk3 is a metabolic checkpoint regulator in B cells
    Authors: J Jellusova, MH Cato, JR Apgar, P Ramezani-R, CR Leung, C Chen, AD Richardson, EM Conner, RJ Benschop, JR Woodgett, RC Rickert
    Nat. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. BCR and Endosomal TLR Signals Synergize to Increase AID Expression and Establish Central B Cell Tolerance
    Authors: M Kuraoka, PB Snowden, T Nojima, L Verkoczy, BF Haynes, D Kitamura, G Kelsoe
    Cell Rep, 2017;18(7):1627-1635.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment
    Authors: A De Riva, M Wållberg, F Ronchi, R Coulson, A Sage, L Thorne, I Goodfellow, KD McCoy, M Azuma, A Cooke, R Busch
    PLoS ONE, 2017;12(8):e0181964.
    Species: Mouse
    Sample Type:
    Application: ELISA Standard
  4. PD-L2 Regulates B-1 Cell Antibody Production against Phosphorylcholine through an IL-5-Dependent Mechanism
    Authors: JT McKay, MA Haro, CA Daly, RD Yammani, B Pang, WE Swords, KM Haas
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

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