Recombinant Mouse beta-NGF Protein

Carrier Free

Catalog # Availability Size / Price Qty
1156-NG-100/CF

With Carrier

Catalog # Availability Size / Price Qty
1156-NG-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (16)
FAQs
Reviews (1)

Recombinant Mouse beta-NGF Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.1-1 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
Ser122-Gly241
Accession #
N-terminal Sequence
Analysis
Ser122
Predicted Molecular Mass
13.5 kDa

Product Datasheets

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1156-NG (with carrier)

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1156-NG/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1156-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1156-NG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: beta-NGF

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

References
  1. Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
  2. Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
  3. Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
  4. Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
  5. Angeletti, R H. et al. (1973) Biochemistry 12:100.
  6. Ullrich, A. et al. (1983) Nature 303:821.
  7. Gotz, R. et al. (1994) Nature 372:266.
  8. Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
  9. Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
  10. E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.
Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Citations for Recombinant Mouse beta-NGF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types
    Authors: J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt
    PLoS ONE, 2022;17(7):e0269818.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons
    Authors: P Pagella, J Catón, CT Meisel, TA Mitsiadis
    Cells, 2020;9(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence
    Authors: Y Zhai, G Yao, F Rao, Y Wang, X Song, F Sun
    Reprod. Biol. Endocrinol., 2018;16(1):28.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A pivotal role of BEX1 in liver progenitor cell expansion in mice
    Authors: Y Gu, W Wei, Y Cheng, B Wan, X Ding, H Wang, Y Zhang, M Jin
    Stem Cell Res Ther, 2018;9(1):164.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors
    Authors: F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu
    Sci Rep, 2017;7(0):41270.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Epiregulin and EGFR interactions are involved in pain processing
    Authors: LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction
    PLoS ONE, 2016;11(8):e0160548.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Knockdown of TrkA in cumulus oocyte complexes (COCs) inhibits EGF-induced cumulus expansion by down-regulation of IL-6.
    Authors: Wang Y, Liang N, Yao G, Tian H, Zhai Y, Yin Y, Sun F
    Mol Cell Endocrinol, 2014;382(2):804-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Treating small fiber neuropathy by topical application of a small molecule modulator of ligand-induced GFRalpha/RET receptor signaling.
    Authors: Hedstrom K, Murtie J, Albers K, Calcutt N, Corfas G
    Proc Natl Acad Sci U S A, 2014;111(6):2325-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
    Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
    PLoS ONE, 2014;9(2):e89881.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism.
    Authors: Howard, Laura, Wyatt, Sean, Nagappan, Guhan, Davies, Alun M
    Development, 2013;140(10):2108-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum).
    Authors: Satoh A, Cummings GM, Bryant SV, Gardiner DM
    Dev. Biol., 2010;337(2):444-57.
    Species: Amphibian
    Sample Types: In Vivo
    Applications: In Vivo
  15. Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
    Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
    PLoS ONE, 2010;5(12):e15053.
  16. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Mouse beta-NGF Protein
By Laura Howard on 11/22/2019
Application: In vitro bioactivity in cell culture
Reason for Rating: Potent promoter of survival and outgrowth of sympathetic neurons

Dissected P0 mouse SCG neurons grown overnight with 10ng/ml NGF and stained with calcein-AM. Images captured by fluorescent microscopy.