Recombinant Mouse beta-NGF Protein

R&D Systems | Catalog # 1156-NG

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse beta-NGF Protein (1156-NG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q6LDU8

Applications

Bioactivity
View all beta-NGF Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
Ser122-Gly241

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser122

Predicted Molecular Mass

13.5 kDa

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.1-1 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 1156-NG in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1156-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1156-NG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-NGF

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

References

  1. Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
  2. Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
  3. Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
  4. Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
  5. Angeletti, R H. et al. (1973) Biochemistry 12:100.
  6. Ullrich, A. et al. (1983) Nature 303:821.
  7. Gotz, R. et al. (1994) Nature 372:266.
  8. Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
  9. Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
  10. E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Gene Symbol

NGF

UniProt

Q6LDU8

Additional beta-NGF Products

Product Documents for Recombinant Mouse beta-NGF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1156-NG (with carrier)
Download SDS 1156-NG/CF (carrier free)

Product Specific Notices for Recombinant Mouse beta-NGF Protein

For research use only

Related Research Areas

Diabetic Peripheral Neuropathy

Citations for Recombinant Mouse beta-NGF Protein

Customer Reviews for Recombinant Mouse beta-NGF Protein (1)

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  • Recombinant Mouse beta-NGF Protein
    Name: Laura Howard
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/22/2019
    Dissected P0 mouse SCG neurons grown overnight with 10ng/ml NGF and stained with calcein-AM. Images captured by fluorescent microscopy.
    Recombinant Mouse beta-NGF Protein 1156-NG

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