Recombinant Mouse BMP-7 Protein

R&D Systems | Catalog # 5666-BP

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse BMP-7 Protein (5666-BP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_031583

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BMP-7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BMP-7 protein
Ser292-His430

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser292

Predicted Molecular Mass

15.6 kDa (monomer)

SDS-PAGE

16-20 kDa under reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.125-0.5 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 5666-BP in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 5666-BP
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 5666-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-7

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Mouse BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 262 aa propeptide, and a 139 aa growth factor domain (3). The growth factor domain of mouse BMP-7 shares 98% and 100% aa sequence identity with human and rat BMP-7, respectively. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (4, 5). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (6-8). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (5). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (4). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 5). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (9, 10), inhibits the branching of prostate epithelium (11), and antagonizes epithelial-mesenchymal transition (EMT) (12-14). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (13), ameliorates fibrotic damage in nephritis (12), and promotes neuroregeneration following brain ischemia (15).

References

  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1991) Biochem. Biophys. Res. Commun. 179:116.
  4. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  5. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  6. Israel, D.I. et al. (1996) Growth Factors 13:291.
  7. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  8. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  9. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  10. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  11. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  12. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  13. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  14. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  15. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Gene Symbol

BMP7

UniProt

NP_031583 (Mouse)

Additional BMP-7 Products

Product Documents for Recombinant Mouse BMP-7 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 5666-BP (with carrier)
Download SDS 5666-BP/CF (carrier free)

Product Specific Notices for Recombinant Mouse BMP-7 Protein

For research use only

Citations for Recombinant Mouse BMP-7 Protein

Customer Reviews for Recombinant Mouse BMP-7 Protein (1)

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  • Recombinant Mouse BMP-7 Protein
    Name: Andres Ocampo
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/12/2022
    mBMP7 protein was used to inhibit proliferation of RM-9-CRL-3312 mouse prostate cancer cells. For this, cells were treated with 1 µg/mL for 24 h, and their proliferation using Ki67 ELISA was tested, comparing the cells against untreated cells. A significantly increase in Ki67 expression was observed in the BMP7-treated cells.
    Recombinant Mouse BMP-7 Protein 5666-BP

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