Recombinant Mouse BTNL4 Fc Chimera Protein, CF

R&D Systems | Catalog # 9590-BT

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse BTNL4 Fc Chimera Protein (9590-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_109671

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BTNL4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTNL4 protein
Mouse BTNL4
(Gln29-Trp250)
Accession # NP_109671		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln29 inferred from enzymatic pyroglutamate treatment revealing Glu30

Predicted Molecular Mass

52 kDa

SDS-PAGE

59-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 1.5-7.5 μg/mL.

Scientific Data Images for Recombinant Mouse BTNL4 Fc Chimera Protein, CF

Recombinant Mouse BTNL4 Fc Chimera Protein Bioactivity

Recombinant Mouse BTNL4 Fc Chimera Protein Bioactivity

Recombinant Mouse BTNL4 Fc Chimera (Catalog # 9590-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1.5-7.5 μg/mL.

Formulation, Preparation, and Storage

9590-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL4

Butyrophilin-like 4, also known as BTLN4, BTN3A3 (human) and BTN3A2 (rat) is an approximately 65 kDa member of the butyrophilin family that may play a role in immune regulation (1-3). The mouse BTNL4 is a 586 aa type 1 transmembrane protein, consisting of a 28 aa signal peptide, followed by a 109 aa immunoglobulin (Ig) V domain, a 71 aa IgC domain, a 20 aa transmembrane domain, and a 170 aa cytoplasmic B30.2/SPRY domain (4). Within the extracellular domain including aa 29‑250, mouse BTNL4 shares 45% and 82% sequence identity with human BTN3A3 and rat BTN3A2, respectively. BTNL4 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL4 co-inhibited anti-CD3 induced IL‑2 secretion on CD3+ cells.

References

  1. Yamashiro, H. et al. (2010) J. Leukoc Biol. 88:757.
  2. Compte, E. et al. (2004) Eur. J. Immunol. 34:2089.
  3. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  4. Bas, A. et al. (2011) Proc Natl Acad Sci U S A. 108:4376.

Long Name

Butyrophilin-like 4

Alternate Names

BTN3A3, EG632126, NG11

Entrez Gene IDs

632126 (Mouse)

Gene Symbol

BTNL4

UniProt

NP_109671

Additional BTNL4 Products

Product Documents for Recombinant Mouse BTNL4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse BTNL4 Fc Chimera Protein, CF

For research use only

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