Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

R&D Systems | Catalog # 5658-CE

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Carboxyl Ester Lipase/CEL Protein (5658-CE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q64285

Structure / Form

Monomer

Applications

Enzyme Activity
View all Carboxyl Ester Lipase/CEL Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Carboxyl Ester Lipase/CEL protein
Ala21-Leu534, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

58 kDa

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to cleave p-Nitrophenyl butyrate (PNPB).
The specific activity is >4,000 pmol/min/μg, as measured under the described conditions. 

Formulation, Preparation, and Storage

5658-CE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Carboxyl Ester Lipase/CEL

Carboxyl ester lipase, also known as bile salt-stimulated lipase, belongs to the Type-B carboxyl esterase/lipase family of enzymes. The enzyme possesses a Ser‑His‑Asp catalytic triad, and is capable of hydrolyzing both ester and amide bonds. CEL is a non-specific lipase which hydrolyzes a variety of substrates, including cholesteryl esters, acylglycerols, and ceramide (1). CEL is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract, where it contributes to the digestion of dietary lipids (2). It is expressed at much lower levels by the liver, macrophages, and endothelial cells (3). CEL is present in the circulation, where it may play a role in low density lipoprotein metabolism and atherogenesis (4).

References

  1. Wang, C.S. and J.A. Hartsuck, J.A. (1993) Biochim. Biophys. Acta. 1166:1.
  2. Lombardo, D. (2001) Biochim. Biophys. Acta. 1533:1.
  3. Hui, D.Y. and Howles, P.N. (2002) J. Lipid Res. 43:2017.
  4. Brodt-Eppley, J. et al. (1995) Biochim. Biophys. Acta. 1272:69.

Alternate Names

BAL, BSDL, BSSL, CEL, FAPP

Entrez Gene IDs

1056 (Human); 12613 (Mouse); 24254 (Rat)

Gene Symbol

CEL

UniProt

Q64285

Additional Carboxyl Ester Lipase/CEL Products

Product Documents for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

For research use only

Related Research Areas

Bioactive Lipids and Receptors

Citations for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

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Protocols

View specific protocols for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF (5658-CE):

Materials
  • Assay Buffer: 25 mM Tris, pH 7.0
  • Reading Buffer: 25 mM Tris, pH 8.0
  • Recombinant Mouse Carboxyl Ester Lipase/CEL (rmCEL) (Catalog # 5658-CE)
  • Substrate: p-Nitrophenyl butyrate (PNPB) (Sigma, Catalog # N9876), 100 mM stock in acetone
  • 96-well Clear Plate (Catalog # DY990)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmCEL to 0.2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 2 mM in Assay Buffer.
  3. Load into the wells of a clear microplate 50 μL of 0.2 ng/µL rmCEL, and start the reaction by adding 50 µL of Substrate. Include a Blank Control containing 50 µL of Assay Buffer and 50 µL of Substrate without any rmCEL.
  4. Incubate 10 minutes at room temperature.
  5. Quickly add 100 µL of Reading Buffer to all wells (this will not stop the reaction).
  6. Read immediately at a wavelength of 410 nm (bottom read) in endpoint mode.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).

Per Well:

  • rmCEL: 0.010 µg
  • Substrate: 0.5 mM

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