Recombinant Mouse Caspr2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10196-CR-100
Recombinant Mouse Caspr2 His-tag Protein, CF SDS-PAGE
1 Image
Product Details
FAQs
Reviews

Recombinant Mouse Caspr2 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Contactin-1 Fc Chimera (Catalog # 904-CN) that produces 50% of the optimal binding response is 5-30 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Caspr2 protein
Ala28-Ser1262, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala28
Predicted Molecular Mass
138 kDa
SDS-PAGE
130-150 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10196-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 130-150 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Caspr2

Contactin Associated Protein-Like 2 (CASPR2), also known as Cntnap2, is a neuronal type I transmembrane member of the neurexin family of adhesion molecules (ref). CASPR2, along with CASPR1 (or Cntnap1), is required for radical and longitudinal organization of myelinated axons (1). Mature mouse CASPR2 consists of a large extracellular domain (ECD) containing four Laminin G-like domains as well as two EFG-like domains and a Fibrinogen C-terminal domain, a transmembrane segment, and a short cytoplasmic domain. The ECD of mouse CASPR2 shares 94% and 93% amino acid (aa) identity with the human and rat ECD, respectively. CASPR2 is thought to be important for the control of neuron-glia interactions and is associated with alterations in synaptic structure, neuronal network activity, and an autism-related phenotype in mice (2, 3). In myelinated nerves, CASPR2 is confined to the juxtaparanodal region of the axon where it appears to associate with the immunoglobulin domains of TAG-1 (transient axonal glycoprotein 1) to form a scaffold, which clusters the potassium channels Kv1.1 and Kv1.2 (4). Recent studies demonstrate that CASPR2 binds to contactin1 (5).

References
  1. Gordon A. et al. (2014) J. Neurosci. 10:1523.
  2. Cope, E. et al. (2016) eNeuro. 0196-16.
  3. Peñagarikano O. et al. (2011) Cell. 147(1):236.
  4. Poliak, S. et al. (1999) Neuron 24:1037.
  5. Rubio-Marrero, E. N. et al. (2016) J. Bio. Chem. 291:5788.
Long Name
Contactin Associated Protein-like 2
Entrez Gene IDs
26047 (Human); 66797 (Mouse)
Alternate Names
AUTS15; Caspr2; CASPR2DKFZp781D1846; CDFE; Cell recognition molecule Caspr2; CNTNAP2; contactin associated protein-like 2; contactin-associated protein 2; contactin-associated protein-like 2; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; NRXN4Caspr2; PTHSL1

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse Caspr2 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Caspr2 His-tag Protein, CF and earn rewards!

Have you used Recombinant Mouse Caspr2 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review