Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).
Recombinant Mouse Cathepsin C/DPPI Protein, CF
R&D Systems | Catalog # 1034-CY
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Cathepsin C/DPPI Protein (1034-CY)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cathepsin C/DPPI protein
Asp25-Leu462, with a C-terminal 10-His tag
Accession # P97821
Asp25-Leu462, with a C-terminal 10-His tag
Accession # P97821
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp25
Predicted Molecular Mass
51 kDa
SDS-PAGE
52 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Arg-7-amido-4-methylcoumarin (GR-AMC).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
1034-CY
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin C/DPPI
References
- Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 1192, Academic Press, San Diego.
- Dahl, S.W. et al. (2001) Biochemistry 40:1671.
Alternate Names
CTSC, DPPI, PALS, PLS
Gene Symbol
CTSC
UniProt
Additional Cathepsin C/DPPI Products
Product Documents for Recombinant Mouse Cathepsin C/DPPI Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Cathepsin C/DPPI Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse Cathepsin C/DPPI Protein, CF
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Protocols
View specific protocols for Recombinant Mouse Cathepsin C/DPPI Protein, CF (1034-CY):
Materials
- Activation Buffer: 25 mM MES, 5 mM DTT, pH 5.5
- Assay Buffer: 25 mM MES, 50 mM NaCl, 5 mM DTT, pH 6.0
- Recombinant Mouse Cathepsin C/DPPI (rmCathepsin C) (Catalog # 1034-CY)
- Recombinant Human Cathepsin L (rhCathepsin L) (Catalog # 952-CY)
- Substrate: Gly-Arg-AMC (Bachem, Catalog # I-1215)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmCathepsin C to 20 µg/mL in Activation Buffer.
- Dilute rhCathepsin L to 2 µg/mL in Activation Buffer.
- Combine equal volumes of diluted rmCathepsin C and diluted rhCathepsin L for final concentrations of 10 µg/mL rmCathepsin C and 1 µg/mL for rhCathepsin L.
- Incubate at room temperature for 20 minutes to activate rmCathepsin C.
- Dilute activated rmCathepsin C to 0.04 ng/µL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- Load 50 µL of the 0.04 ng/µL rmCathepsin C in a plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A9891).
Per Well:
- rmCathepsin C: 0.002 µg
- Substrate: 10 µM