Recombinant Mouse CCL25/TECK Protein

Carrier Free

Catalog # Availability Size / Price Qty
481-TK-025/CF

With Carrier

Catalog # Availability Size / Price Qty
481-TK-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (17)
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Recombinant Mouse CCL25/TECK Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR9. The ED50 for this effect is 0.1-0.5 µg/mL.
Source
E. coli-derived mouse CCL25/TECK protein
Gln24-Asn144, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
14 kDa
SDS-PAGE
16-17 kDa doublet, reducing conditions

Product Datasheets

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481-TK (with carrier)

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481-TK/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

481-TK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

481-TK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CCL25/TECK

CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature mouse CCL25 shares 40% and 81% amino acid sequence identity with human and rat CCL25, respectively (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7+ gamma δ T cells (6, 7), Integrin alpha E beta 7+ CD8+ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques (14).

References
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
  2. Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
  3. Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
  4. Zaballos, A. et al. (1999) J. Immunol. 162:5671.
  5. Youn, B.-S. et al. (1999) Blood 94:2533.
  6. Uehara, S. et al. (2002) J. Immunol. 168:134.
  7. Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
  8. Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
  9. Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
  10. Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
  11. Xu, S. et al. (2012) Stem Cells 30:266.
  12. Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
  13. Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
  14. Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.
Entrez Gene IDs
6370 (Human); 20300 (Mouse)
Alternate Names
C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine

Citations for Recombinant Mouse CCL25/TECK Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Downregulation of chemokine receptor 9 facilitates CD4+CD8??+ intraepithelial lymphocyte development
    Authors: Ono, K;Sujino, T;Miyamoto, K;Harada, Y;Kojo, S;Yoshimatsu, Y;Tanemoto, S;Koda, Y;Zheng, J;Sayama, K;Koide, T;Teratani, T;Mikami, Y;Takabayashi, K;Nakamoto, N;Hosoe, N;London, M;Ogata, H;Mucida, D;Taniuchi, I;Kanai, T;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Chemokine receptor CCR9 suppresses the differentiation of CD4+CD8alphaalpha+ intraepithelial T cells in the gut
    Authors: C Li, HK Kim, P Prakhar, S Luo, A Crossman, DL Ligons, MA Luckey, P Awasthi, RE Gress, JH Park
    Mucosal Immunology, 2022-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The murine DCs transfected with DNA-plasmid encoding CCR9 demonstrate the increased migration to CCL25 and thymic cells in vitro and to the thymus in vivo
    Authors: V Tereshchen, A Bulygin, R Zavodskii, A Maksyutov, V Kurilin, M Fisher, N Semenyuk, S Aladev, S Sennikov
    Cytokine, 2021-02-26;142(0):155473.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A mutation that blocks integrin alpha4beta7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis
    Authors: H Zhang, Y Zheng, Y Pan, C Lin, S Wang, Z Yan, L Lu, G Ge, J Li, YA Zeng, J Chen
    BMC Biol., 2020-06-10;18(1):64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Insights into ligand binding by a viral tumor necrosis factor (TNF) decoy receptor yield a selective soluble human type 2 TNF receptor
    Authors: SM Pontejo, C Sanchez, B Ruiz-Argüe, A Alcami
    J. Biol. Chem., 2019-02-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
    Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
    Elife, 2018-09-06;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
    Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
    Nat. Methods, 2017-04-10;14(5):531-538.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting.
    Authors: Chen H, Sun J, Huang Z, Hou H, Arcilla M, Rakhilin N, Joe D, Choi J, Gadamsetty P, Milsom J, Nandakumar G, Longman R, Zhou X, Edwards R, Chen J, Chen K, Bu P, Wang L, Xu Y, Munroe R, Abratte C, Miller A, Gumus Z, Shuler M, Nishimura N, Edelmann W, Shen X, Lipkin S
    Nat Biotechnol, 2015-05-25;33(6):656-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease.
    Authors: Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B
    PLoS ONE, 2014-12-01;9(12):e112925.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. PTEN action in leukaemia dictated by the tissue microenvironment.
    Authors: Miething, Corneliu, Scuoppo, Claudio, Bosbach, Benedikt, Appelmann, Iris, Nakitandwe, Joy, Ma, Jing, Wu, Gang, Lintault, Laura, Auer, Martina, Premsrirut, Prem K, Teruya-Feldstein, Julie, Hicks, James, Benveniste, Helene, Speicher, Michael, Downing, James R, Lowe, Scott W
    Nature, 2014-05-04;510(7505):402-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
    Authors: McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C
    Immunity, 2011-04-22;34(4):602-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Involvement of CC chemokines in gammadelta T lymphocyte trafficking during allergic inflammation: the role of CCL2/CCR2 pathway.
    Authors: Penido C, Costa MF, Souza MC, Costa KA, Candea AL, Benjamim CF, Henriques MG
    Int. Immunol., 2007-12-04;20(1):129-39.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007-09-15;179(6):3724-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut.
    Authors: Park EJ, Mora JR, Carman CV, Chen J, Sasaki Y, Cheng G, von Andrian UH, Shimaoka M
    J. Clin. Invest., 2007-09-01;117(9):2526-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
    Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
    J. Immunol., 2004-09-15;173(6):3668-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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