Recombinant Mouse CD160 Protein, CF Summary
When Recombinant Mouse HVEM Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD160 that produces 50% of the optimal binding response is found to be approximately 0.012 ‑ 0.06 μg/mL.
Gly28-Ser160 with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD160 (also Natural killer cell receptor BY55) is a 16 kDa (predicted) member of the Ig superfamily (1 ‑ 4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ TEM and TCM T cells, CD8 alpha + IELs, NKT cells, CD8-gamma δ TCR T cells, and vascular endothelial cells (1, 5 - 7). Mouse CD160 has been identified as a 20 - 21 kDa GPI-linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185 amino acids (aa) in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V-type Ig-like domain (aa 28 - 125), and a 25 aa prosegment that is cleaved to generate a GPI-linkage at Ser160. Mouse GPI-linked CD160 is known to be cleaved by phospholipase C, and this generates a 40 kDa (presumably dimeric) band in SDS-PAGE (5). One alternative splice form for mouse CD160 is reported that appears to show a deletion of aa 137 - 180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively.
In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non‑classical MHC Class I molecules (5, 8). MHC‑I proteins recognized by CD160 include Dd, Kb, Qa-1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN-gamma secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).
- Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
- del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
- Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
- Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
- Maeda, M. et al. (2005) J. Immunol. 175:4426.
- Fons, P. et al. (2006) Blood 108:2608.
- Tsujimura, K. et al. (2006) Immunol. Lett. 48:106.
- Cheung, T.C. et al. (2009) Proc. Natl. Acad. Sci USA 106:6244.
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