Recombinant Mouse CD1d1 Fc Chimera Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
(Gln22 - Gly305)
(Glu98 - Lys330)
beta 2-microglobulin from mouse myeloma cells
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD1d is a 48 kDa transmembrane glycoprotein that belongs to the CD1 family of glycolipid antigen-presenting MHC-like molecules. In mouse, there are two closely related CD1d genes, CD1d1 and CD1d2, whereas human and rat have only one (1, 2). Mature mouse CD1d1 consists of a 284 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail (3). Within the ECD, mouse CD1d1 shares 94% aa sequence identity with mouse CD1d2, and 65% and 87% with human and rat CD1d, respectively. Complexes of CD1d1 with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). A cytoplasmic motif mediates CD1d1 recycling through the endosomal/lysosomal system where it is loaded with processed exogenous glycolipids by saposin lipid transfer proteins (4 - 8). CD1d1-presented glycolipids are recognized by canonical NKT cells that utilize an invariant V alpha 14-J alpha 18 chain in their T cell receptor (V alpha 24-J alpha 18 in human) (9, 10). NKT cells that express V chains other than alpha 14 can also recognize CD1d1-presented glycolipids but do not require them to be endosomally loaded (10, 11). NKT cells respond to a variety of CD1d1-presented glycolipids including alpha -galactosylceramide ( alpha -GalCer), a structural relative of microbial cell wall components, and the endogenous isoglobotrihexosylceramide (iGb3) (2, 9, 12). The interaction with glycolipid-loaded CD1d1 is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (10, 11, 13, 14). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (15).
- Bendelac, A. et al. (2007) Annu. Rev. Immunol. 25:297.
- Brutkiewicz, R.R. (2006) J. Immunol. 177:769.
- Balk, S.P. et al. (1991) J. Immunol. 146:768.
- De Silva, A.D. et al. (2002) J. Immunol. 168:723.
- Roberts, T.J. et al. (2002) J. Immunol. 168:5409.
- Kang, S.J. and P. Cresswell (2004) Nat. Immunol. 5:175.
- Zhou, D. et al. (2004) Science 303:523.
- Prigozy, T.O. et al. (2001) Science 291:664.
- Kawano, T. et al. (1997) Science 278:1626.
- Chiu, Y.H. et al. (1999) J. Exp. Med. 189:103.
- Behar, S.M. et al. (1999) J. Immunol. 162:161.
- Zhou, D. et al. (2004) Science 306:1786.
- Mendiratta, S.K. et al. (1997) Immunity 6:469.
- Stanic, A.K. et al. (2003) J. Immunol. 171:4539.
- Yuan, W. et al. (2006) Nat. Immunol. 7:835.
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